Seo Yeeun, Hossain Md Iqbal, Wang Zhaoqi, Yeo Daseul, Jung Soontag, Woo Seoyoung, Zhang Yuan, Rhee Min Suk, Choi Changsun
Department of Food and Nutrition, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Gyeonggi-do 17546, Republic of Korea.
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Transbound Emerg Dis. 2024 Jan 29;2024:8131727. doi: 10.1155/2024/8131727. eCollection 2024.
Tick-borne virus detection in livestock and slaughterhouse animals has recently surged in the United States and Europe. Although cases of patients with tick-borne illnesses have been reported in Korea, food contamination from tick-borne viruses has yet to be investigated. Therefore, this study investigated severe fever with thrombocytopenia syndrome virus (SFTSV), tick-borne encephalitis virus (TBEV), and Crimean-Congo hemorrhagic fever virus (CCHFV) prevalence in meat and dairy products. A total of 628 products were collected from a Korean retail market during 2021-2022, including 195 beef, 130 goats, 90 lambs, 61 pork, 50 chicken, and 38 commercial cheese samples. In addition, 64 raw cow milk samples were collected from a ranch in Anseong-si, Gyeonggi-do, from 2021 to 2022. Real-time reverse transcription-polymerase chain reaction (RT-PCR), nested reverse transcription polymerase chain reaction (RT-nPCR), virus cultivation, and sequence analysis were conducted. SFTSV was detected in 1.53% (3/195) beef and 0.76% (1/130) goat meat samples with a low Ct value titer from 33.18 to 38.60. In contrast, SFTSV was neither detected in lamb, pork, chicken, raw milk, or cheese samples nor were TBEV and CCHFV detected in any of the tested samples. Although no existing cases or studies have indicated SFTSV transmittance through food, this study confirmed SFTSV genotype B RNA in SFTSV-positive meat samples. Therefore, monitoring for and evaluating SFTSV-contaminated meat products must be investigated in future studies.
美国和欧洲近期家畜及屠宰场动物中蜱传病毒检测数量激增。尽管韩国已报告蜱传疾病患者病例，但蜱传病毒对食品的污染情况尚未得到调查。因此，本研究调查了肉类和乳制品中严重发热伴血小板减少综合征病毒（SFTSV）、蜱传脑炎病毒（TBEV）和克里米亚-刚果出血热病毒（CCHFV）的流行情况。2021年至2022年期间，从韩国一个零售市场共采集了628份产品，包括195份牛肉、130份山羊肉、90份羔羊肉、61份猪肉、50份鸡肉和38份商业奶酪样品。此外，2021年至2022年期间，从京畿道安城市的一个牧场采集了64份生牛奶样品。进行了实时逆转录-聚合酶链反应（RT-PCR）、巢式逆转录聚合酶链反应（RT-nPCR）、病毒培养和序列分析。在1.53%（3/195）的牛肉和0.76%（1/130）的山羊肉样品中检测到SFTSV，其低Ct值滴度为33.18至38.60。相比之下，在羔羊肉、猪肉、鸡肉、生牛奶或奶酪样品中均未检测到SFTSV，在任何测试样品中也未检测到TBEV和CCHFV。尽管目前没有病例或研究表明SFTSV可通过食物传播，但本研究在SFTSV阳性肉类样品中证实了SFTSV B基因型RNA。因此，未来的研究必须对受SFTSV污染的肉类产品进行监测和评估。
