Virginia Tech-Wake Forest School of Biomedical Engineering and Sciences, Bioelectromechanical Systems Laboratory, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
IEEE Trans Biomed Eng. 2011 May;58(5):1474-82. doi: 10.1109/TBME.2010.2102021. Epub 2010 Dec 23.
This study introduces the use of high-frequency pulsed electric fields for tissue electroporation. Through the development of finite element models and the use of analytical techniques, electroporation with rectangular, bipolar pulses is investigated. The electric field and temperature distribution along with the associated transmembrane potential development are considered in a heterogeneous skin fold geometry. Results indicate that switching polarity on the nanosecond scale near the charging time of plasma membranes can greatly improve treatment outcomes in heterogeneous tissues. Specifically, high-frequency fields ranging from 500 kHz to 1 MHz are best suited to penetrate epithelial layers without inducing significant Joule heating, and cause electroporation in underlying cells.
本研究介绍了高频脉冲电场在组织电穿孔中的应用。通过开发有限元模型和使用分析技术，研究了矩形双极脉冲的电穿孔。在非均质皮肤褶皱几何形状中，考虑了电场和温度分布以及相关的跨膜电位发展。结果表明，在接近细胞膜充电时间的纳秒尺度上切换极性可以极大地改善非均质地 组织中的治疗效果。具体来说，频率范围在 500 kHz 至 1 MHz 的高频场最适合穿透上皮层而不会引起明显的焦耳加热，并在下面的细胞中引起电穿孔。
