Department of Endodontics, Hamedan University of Medical Sciences, Hamedan, Iran.
Med Oral Patol Oral Cir Bucal. 2011 Jul 1;16(4):e588-92. doi: 10.4317/medoral.16.e588.
In this in vitro study the antibacterial substantivity of a new sodium hypochlorite-based root canal irrigant (Hypoclean) in bovine root dentin was investigated.
Ninety dentin tubes prepared from bovine incisor teeth were used. After contamination for 14 days with Enterococcus faecalis, the specimens were divided into five groups as follows: Hypoclean; Tetraclean; 5.25% sodium hypochlorite (NaOCl); infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected with round burs into tryptic soy broth and after culturing, the number of colony-forming units (CFU) was counted.
The number of CFU was minimum in the first cultures in all experimental groups, and the results obtained were significantly different from each other at any time period (P < 0.05). At all five experimental periods, the Tetraclean group showed the most effective antibacterial action (P < 0.05). Regular 5.25% NaOCl group showed the worst result at all periods. Hypoclean group demonstrated no bacterial growth after treatment. In each group, the number of CFU increased significantly by time-lapse (P < 0.05).
The residual antibacterial activity of Tetraclean was significantly greater than Hypoclean and 5.25% NaOCl.
本体外研究旨在探究一种新型次氯酸钠根管冲洗剂（Hypoclean）在牛牙本质中的抗菌持续性。
本研究共使用了 90 个从牛门牙制备的牙本质管。在被粪肠球菌污染 14 天后，将标本分为以下五组：Hypoclean 组；Tetraclean 组；5.25%次氯酸钠（NaOCl）组；感染牙本质管（阳性对照组）；无菌牙本质管（阴性对照组）。用圆形车针将牙本质屑收集到胰蛋白酶大豆肉汤中，培养后，计算菌落形成单位（CFU）的数量。
在所有实验组的第一次培养中，CFU 的数量最少，且在任何时间段的结果均存在显著差异（P < 0.05）。在所有五个实验期间，Tetraclean 组表现出最有效的抗菌作用（P < 0.05）。常规 5.25% NaOCl 组在所有时期的结果均最差。Hypoclean 组在处理后未检测到细菌生长。在每个组中，CFU 的数量随时间推移显著增加（P < 0.05）。
Tetraclean 的残留抗菌活性明显大于 Hypoclean 和 5.25% NaOCl。
