Giardino Luciano, Mohammadi Zahed, Beltrami Riccardo, Poggio Claudio, Estrela Carlos, Generali Luigi
Private Practice, Crotone, Italy.
Institute of Dental Sciences Shahid Beheshti, University of Medical Sciences, Tehran, Iran.
Braz Dent J. 2016 Jan-Feb;27(1):32-6. doi: 10.1590/0103-6440201600627.
The aim of this study was to compare the antimicrobial activity of 5.25% NaOCl, Hypoclean and Chlor-Xtra at 20 °C and 45 °C in bovine root dentin. One-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with Enterococcus faecalis. The specimens were divided into the following groups: 1. 5.25% NaOCl 20 °C; 2. Hypoclean 20 °C; 3. Chlor-Xtra 20 °C; 4. 5.25% % NaOCl 45 °C; 5. Hypoclean 45 °C; 6. Chlor-Xtra 45 °C; 7. positive control; 8. negative control. Dentin chips were collected with round burs into Brain Heart Infusion (BHI) broth. After culturing, the number of colony-forming units (CFU) was counted. Statistical analyses were performed using descriptive statistics (mean, standard deviation, median), Shapiro-Wilk test, ANOVA and Tukey test. Significance level was set at p<0.05. In all experimental groups, CFU was minimum after treatment (day 0) and the obtained results were significantly different from each other at any period (p<0.05). After treatment, the Hypoclean and Chlor-Xtra showed the lowest numbers of CFU at 20 °C and 45 °C, whereas 5.25% NaOCl showed the highest number of CFU at both temperatures. In each group, the number of CFUs increased significantly with time (p<0.05). The antibacterial activity of Hypoclean and Chlor-Xtra at 45 °C were significantly greater than other tested solutions.
本研究的目的是比较5.25%次氯酸钠、Hypoclean和Chlor-Xtra在20℃和45℃条件下对牛牙根牙本质的抗菌活性。用牛上颌切牙制备170根管状牙本质，用粪肠球菌感染21天。将标本分为以下几组：1. 5.25%次氯酸钠20℃；2. Hypoclean 20℃；3. Chlor-Xtra 20℃；4. 5.25%次氯酸钠45℃；5. Hypoclean 45℃；6. Chlor-Xtra 45℃；7. 阳性对照；8. 阴性对照。用圆钻收集牙本质碎屑至脑心浸液（BHI）肉汤中。培养后，计算菌落形成单位（CFU）的数量。采用描述性统计（均值、标准差、中位数）、Shapiro-Wilk检验、方差分析和Tukey检验进行统计分析。显著性水平设定为p<0.05。在所有实验组中，处理后（第0天）CFU最少，且在任何时期获得的结果彼此间均有显著差异（p<0.05）。处理后，Hypoclean和Chlor-Xtra在20℃和45℃时显示出最低的CFU数量，而5.25%次氯酸钠在两个温度下均显示出最高的CFU数量。在每组中，CFU数量随时间显著增加（p<0.05）。Hypoclean和Chlor-Xtra在45℃时的抗菌活性显著高于其他测试溶液。
