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磷酸转移酶系统的组氨酸蛋白（HPr）参与大肠杆菌K-12的趋化信号传导。

Involvement of the histidine protein (HPr) of the phosphotransferase system in chemotactic signaling of Escherichia coli K-12.

作者信息

Grübl G, Vogler A P, Lengeler J W

机构信息

Fachbereich BiologielChemie, Universität Osnabrück, Federal Republic of Germany.

出版信息

J Bacteriol. 1990 Oct;172(10):5871-6. doi: 10.1128/jb.172.10.5871-5876.1990.

DOI:10.1128/jb.172.10.5871-5876.1990
PMID:2120191
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC526906/
Abstract

It is known that in mutants of Escherichia coli lacking the histidine protein (HPr) of the carbohydrate: phosphotransferase system, all substrates of the system can be taken up in the presence of the fructose-regulated HPr-like protein FPr (gene fruF). Although this protein fully substituted for HPr in transport and phosphorylation, we found that it was not able to complement efficiently for HPr in mediating chemotaxis toward phosphotransferase system substrates. Furthermore, transport activity and chemotaxis could be genetically dissected by the exchange of single amino acids in HPr. The results suggest a specific role of HPr in chemotactic signaling. We propose a possible link of signal transduction pathways for phosphotransferase system- and methyl chemotaxis protein-dependent substrates via HPr.

摘要

已知在缺乏碳水化合物

磷酸转移酶系统的组氨酸蛋白（HPr）的大肠杆菌突变体中，该系统的所有底物在果糖调节的类HPr蛋白FPr（基因fruF）存在的情况下都能被摄取。尽管这种蛋白质在转运和磷酸化过程中完全替代了HPr，但我们发现它在介导对磷酸转移酶系统底物的趋化作用中不能有效地补充HPr。此外，通过HPr中单个氨基酸的交换，可以对转运活性和趋化作用进行遗传分析。结果表明HPr在趋化信号传导中具有特定作用。我们提出了通过HPr的磷酸转移酶系统和甲基趋化蛋白依赖性底物的信号转导途径之间的可能联系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea7/526906/c119cb01a321/jbacter00164-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea7/526906/dfd212b84718/jbacter00164-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea7/526906/c119cb01a321/jbacter00164-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea7/526906/dfd212b84718/jbacter00164-0365-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ea7/526906/c119cb01a321/jbacter00164-0366-a.jpg

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The roles of HPr and FPr in the utilization of fructose by Escherichia coli.HPr和FPr在大肠杆菌利用果糖过程中的作用。
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The phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system enzymes II as chemoreceptors in chemotaxis of Escherichia coli K 12.磷酸烯醇丙酮酸依赖性碳水化合物：磷酸转移酶系统的酶II作为大肠杆菌K12趋化作用中的化学感受器。
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本文引用的文献

1
The phosphoenolpyruvate-dependent carbohydrate: phosphotransferase system enzymes II as chemoreceptors in chemotaxis of Escherichia coli K 12.磷酸烯醇丙酮酸依赖性碳水化合物：磷酸转移酶系统的酶II作为大肠杆菌K12趋化作用中的化学感受器。
Mol Gen Genet. 1981;183(1):163-70. doi: 10.1007/BF00270156.
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Selection for loss of tetracycline resistance by Escherichia coli.大肠杆菌对四环素抗性丧失的选择。
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[Mapping of the mutations within the genes coding for enzyme I and protein Hpr of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli K-12. II. Mapping of the mutations within gene ptsH].
Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic.
聚（3-羟基链烷酸酯）的代谢工程：从DNA到塑料
Microbiol Mol Biol Rev. 1999 Mar;63(1):21-53. doi: 10.1128/MMBR.63.1.21-53.1999.
4
Coupling the phosphotransferase system and the methyl-accepting chemotaxis protein-dependent chemotaxis signaling pathways of Escherichia coli.将大肠杆菌的磷酸转移酶系统与甲基受体趋化蛋白依赖性趋化信号通路相偶联。
Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11583-7. doi: 10.1073/pnas.92.25.11583.
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Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria.细菌的磷酸烯醇丙酮酸：碳水化合物磷酸转移酶系统
Microbiol Rev. 1993 Sep;57(3):543-94. doi: 10.1128/mr.57.3.543-594.1993.
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The Escherichia coli mannitol permease as a model for transport via the bacterial phosphotransferase system.大肠杆菌甘露醇通透酶作为通过细菌磷酸转移酶系统进行转运的模型。
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Change in direction of flagellar rotation in Escherichia coli mediated by acetate kinase.乙酸激酶介导的大肠杆菌鞭毛旋转方向的改变
J Bacteriol. 1993 May;175(10):3236-9. doi: 10.1128/jb.175.10.3236-3239.1993.
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Functions of the gene products of Escherichia coli.大肠杆菌基因产物的功能。
Microbiol Rev. 1993 Dec;57(4):862-952. doi: 10.1128/mr.57.4.862-952.1993.
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Identification and characterization of two Alcaligenes eutrophus gene loci relevant to the poly(beta-hydroxybutyric acid)-leaky phenotype which exhibit homology to ptsH and ptsI of Escherichia coli.与聚（β-羟基丁酸）渗漏表型相关的两个真养产碱菌基因位点的鉴定与表征，这两个位点与大肠杆菌的ptsH和ptsI具有同源性。
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Is cross regulation by phosphorylation of two-component response regulator proteins important in bacteria?双组分反应调节蛋白的磷酸化交叉调节在细菌中重要吗？
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[大肠杆菌K-12中磷酸烯醇丙酮酸依赖性磷酸转移酶系统的酶I和蛋白Hpr编码基因内突变的定位。II. ptsH基因内突变的定位]
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Sensory transducers of E. coli are composed of discrete structural and functional domains.大肠杆菌的感官转导器由离散的结构和功能域组成。
Cell. 1983 Jun;33(2):615-22. doi: 10.1016/0092-8674(83)90442-7.
5
Adenylate cyclase is required for chemotaxis to phosphotransferase system sugars by Escherichia coli.腺苷酸环化酶是大肠杆菌对磷酸转移酶系统糖类进行趋化作用所必需的。
J Bacteriol. 1983 Mar;153(3):1187-95. doi: 10.1128/jb.153.3.1187-1195.1983.
6
Involvement of cyclic GMP in intracellular signaling in the chemotactic response of Escherichia coli.环鸟苷酸参与大肠杆菌趋化反应中的细胞内信号传导。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3879-83. doi: 10.1073/pnas.77.7.3879.
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The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system.细菌磷酸转移酶系统中酶I和热稳定蛋白突变体的生理行为。
J Biol Chem. 1970 Nov 10;245(21):5870-3.
8
A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli.一种测量趋化性的方法以及使用该方法确定大肠杆菌趋化性最佳条件的应用。
J Gen Microbiol. 1973 Jan;74(1):77-91. doi: 10.1099/00221287-74-1-77.
9
Phosphotransferase-system enzymes as chemoreceptors for certain sugars in Escherichia coli chemotaxis.磷酸转移酶系统酶作为大肠杆菌趋化作用中某些糖类的化学感受器。
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2895-9. doi: 10.1073/pnas.71.7.2895.
10
Reversal of the mannitol-sorbitol diauxie in Escherichia coli.大肠杆菌中甘露醇 - 山梨醇二次生长现象的逆转
J Bacteriol. 1972 Nov;112(2):840-8. doi: 10.1128/jb.112.2.840-848.1972.