Pathak Promila, Piri Hossein, Vij S P, Mahant K C, Chauhan Shaveta
Orchid Laboratory, Department of Botany, Panjab University, Chandigarh 160 014, India.
Indian J Exp Biol. 2011 Sep;49(9):711-6.
In vitro asymbiotic seed germination potential of its immature seeds (36 weeks after pollination) of G. calceolaris was successfully tested on three different agar gelled nutrient media i.e. Murashige and Skoog (MS), Mitra et al. (M) and potato dextrose agar (PDA). Seeds germinated within 15.75+/-0.75 to 35.75+/-0.75 days in the three different media. The protocorms developed therefrom subsequently differentiated into first leaf and root primordia, and complete seedlings were obtained within 111.25+/-1.25 to 141.25+/-1.25 days on MS and M media. The protocorms, though failed to differentiate further on basal PDA medium, despite repeated subculturings, incorporation of peptone (P; 1 gl(-1)), yeast extract (YE; 2 gl(-1)) and coconut water (CW; 20%) in the medium proved beneficial in inducing differentiation, in these germinating entities. Additional use of growth additives (P/YE/CW), in general, favoured better germination, protocorm formation and seedling development. The optimal nutritional combination during seed germination, protocorm growth and multiplication and seedling development was found to be CW (10%) enriched MS medium.
在三种不同的琼脂凝胶化营养培养基上，即Murashige和Skoog（MS）培养基、Mitra等人（M）培养基以及马铃薯葡萄糖琼脂（PDA）培养基上，成功测试了荷包花未成熟种子（授粉后36周）的体外非共生种子萌发潜力。种子在三种不同培养基中于15.75±0.75至35.75±0.75天内萌发。由此发育而来的原球茎随后分化为第一片叶子和根原基，在MS和M培养基上于111.25±1.25至141.25±1.25天内获得完整幼苗。尽管在基础PDA培养基上反复继代培养后原球茎未能进一步分化，但在培养基中添加蛋白胨（P；1 g L⁻¹）、酵母提取物（YE；2 g L⁻¹）和椰汁（CW；20%）被证明有利于诱导这些萌发实体的分化。一般来说，额外使用生长添加剂（P/YE/CW）有利于更好的萌发、原球茎形成和幼苗发育。发现在种子萌发、原球茎生长与增殖以及幼苗发育过程中的最佳营养组合是添加10%椰汁的MS培养基。
