Jordt T, Lorenzini E
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Trop Anim Health Prod. 1990 Aug;22(3):178-84. doi: 10.1007/BF02241014.
Five N'Dama heifers were superovulated with follicle stimulating hormone (FSH-P or Folltropin) a total of six times each. The superovulations were carried out between ongoing experimental Trypanosoma congolense infections. Twenty-four (80%) of the 30 superovulations had a good ovarian response with 21 (70%) producing an average of 2.7 +/- 0.4 (mean +/- s.e.m.) embryos. The highest embryo production was achieved at the third and fourth superovulation, after which both the number of embryos and their quality declined. The overall pregnancy rate after transfer into Boran (Bos indicus) cow recipients was 50.9%. The uteri of the heifers increased considerably in size throughout the six superovulations which made it difficult to flush some of the animals after the third superovulation. Embryo transfer technology is a useful breeding tool in N'Dama heifers and multiple superovulations can be carried out with success.
选用5头恩达玛小母牛，每次用促卵泡素（FSH - P或Folltropin）进行超数排卵，每头共进行6次。超数排卵在持续的刚果锥虫感染实验期间进行。30次超数排卵中有24次（80%）卵巢反应良好，其中21次（70%）平均产生2.7±0.4（均值±标准误）枚胚胎。第三次和第四次超数排卵时胚胎产量最高，此后胚胎数量和质量均下降。移植到博拉安（印度瘤牛）母牛受体后的总体妊娠率为50.9%。在整个6次超数排卵过程中，小母牛的子宫大小显著增加，这使得在第三次超数排卵后对部分动物进行胚胎冲洗变得困难。胚胎移植技术是恩达玛小母牛有用的繁殖工具，多次超数排卵可以成功进行。
