Department of Molecular and Microbiology, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA.
J Virol. 2011 Mar;85(6):3020-4. doi: 10.1128/JVI.02462-10. Epub 2011 Jan 5.
Previous studies have observed fluorescently labeled HIV particles tracking along microtubule networks for nuclear localization. To provide direct evidence for the involvement of microtubules in early steps of HIV infection of human CD4 T cells, we used multiple microtubule modulators such as paclitaxel (originally called taxol; 1 μM), vinblastine (1 and 10 μM), colchicine (10 and 100 μM), and nocodazole (10 and 100 μM) to disturb microtubule networks in transformed and resting CD4 T cells. Although these drugs disrupted microtubule integrity, almost no inhibition of HIV-1 infection was observed. Our results do not appear to support an essential role for microtubules in the initiation of HIV infection of CD4 T cells.
先前的研究观察到荧光标记的 HIV 颗粒沿着微管网络追踪到核定位。为了提供微管在 HIV 感染人 CD4 T 细胞的早期步骤中参与的直接证据，我们使用了多种微管调节剂，如紫杉醇（最初称为紫杉醇；1 μM）、长春碱（1 和 10 μM）、秋水仙碱（10 和 100 μM）和诺考达唑（10 和 100 μM），以扰乱转化和静止 CD4 T 细胞中的微管网络。尽管这些药物破坏了微管的完整性，但几乎没有观察到对 HIV-1 感染的抑制。我们的结果似乎不支持微管在 HIV 感染 CD4 T 细胞的起始中起关键作用。
