Italian Inter-University Center for the Study of Electromagnetic Fields and BioSystems (ICEmB) at ENEA, Italian Agency for New Technologies, Energy and Sustainable Economic Development, Rome 00123, Italy.
IEEE Trans Biomed Eng. 2011 May;58(5):1294-302. doi: 10.1109/TBME.2010.2104150. Epub 2011 Jan 6.
A microdosimetric study of nanosecond pulsed electric fields, including dielectric dispersivity of cell compartments, is proposed in our paper. A quasi-static solution based on the Laplace equation was adapted to wideband signals and used to address the problem of electric field estimation at cellular level. The electric solution was coupled with an asymptotic electroporation model able to predict membrane pore density. An initial result of our paper is the relevance of the dielectric dispersivity, providing evidence that both the transmembrane potential and the pore density are strongly influenced by the choice of modeling used. We note the crucial role played by the dielectric properties of the membrane that can greatly impact on the poration of the cell. This can partly explain the selective action reported on cancerous cells in mixed populations, if one considers that tumor cells may present different dielectric responses. Moreover, these kinds of studies can be useful to determine the appropriate setting of nsPEF generators as well as for the design and optimization of new-generation devices.
我们的论文提出了一种纳秒级脉冲电场的微剂量学研究，包括细胞区室的介电弥散性。我们采用基于拉普拉斯方程的准静态解来处理宽带信号，并用于解决细胞水平电场估计的问题。该电场解与能够预测细胞膜孔密度的渐近电穿孔模型相结合。我们论文的初步结果是介电弥散性的相关性，证明了跨膜电位和孔密度都受到所使用的建模选择的强烈影响。我们注意到膜的介电特性所起的关键作用，这可能会极大地影响细胞的穿孔。如果考虑到肿瘤细胞可能具有不同的介电响应，这可以部分解释在混合群体中对癌细胞的选择性作用。此外，这类研究对于确定 nsPEF 发生器的适当设置以及新设备的设计和优化可能是有用的。
