Payal Bhardwaj, Kaur Harkiran Preet, Rai Durg Vijay
Department of Biophysics, Panjab University, Chandigarh, 160014, India.
Interdiscip Toxicol. 2009 Mar;2(1):18-23. doi: 10.2478/v10102-009-0003-5.
Risks of heavy metals-induced severe bone disorders generate interest to their toxicity. The present study was undertaken to monitor the biochemical and antioxidant status of bone of 30 and 80 days old male Wistar rats exposed to 5 week lead treatment. At the end of study, the rats were sacrificed, their long bone i.e. femur were excised, cleaned of soft tissue, minced and homogenized. Nucleic acid content, alkaline phosphatase, lipid peroxidation, catalase, glutathione S-transferase and superoxide dismutase were determined in bone. In both groups of treated animals lead treatment increased the production of malondialdehyde, while reducing activities of catalase, glutathione S-transferase and superoxide dismutase, indicating that it causes oxidative stress. Parallely with these effects lead significantly reduced the nucleic acid content and the activity of alkaline phosphatase, considered as biomarkers of osteoblast's function, conditions and development of bones. Moreover the concentrations of copper, zinc, iron and sodium were reduced in the excised bones. The present study indicates that the lead induced bone toxicity and its deteriorated development is the consequence of a primary oxidative stress. Our results may be helpful in understanding the modulation of biochemical parameters under lead toxicity.
重金属诱发严重骨疾病的风险引发了人们对其毒性的关注。本研究旨在监测经5周铅处理的30日龄和80日龄雄性Wistar大鼠骨骼的生化和抗氧化状态。在研究结束时，处死大鼠，切除其长骨即股骨，清除软组织，切碎并匀浆。测定骨骼中的核酸含量、碱性磷酸酶、脂质过氧化、过氧化氢酶、谷胱甘肽S-转移酶和超氧化物歧化酶。在两组经处理的动物中，铅处理均增加了丙二醛的生成，同时降低了过氧化氢酶、谷胱甘肽S-转移酶和超氧化物歧化酶的活性，表明其会引起氧化应激。与这些效应同时出现的是，铅显著降低了核酸含量和碱性磷酸酶的活性，而核酸含量和碱性磷酸酶活性被视为成骨细胞功能、骨骼状况和发育的生物标志物。此外，切除的骨骼中铜、锌、铁和钠的浓度降低。本研究表明，铅诱导骨毒性及其发育恶化是原发性氧化应激的结果。我们的结果可能有助于理解铅毒性下生化参数的调节。
