College of Biomedical Engineering & Instrument Science, Zhejiang University, 38 Zheda Road, Hangzhou, Zhejiang, China.
J Ethnopharmacol. 2011 Apr 12;134(3):1018-23. doi: 10.1016/j.jep.2011.01.004. Epub 2011 Jan 8.
The present study was conducted to evaluate the anti-inflammatory and antinociceptive activities of Clematis terniflora DC. extracts and fractions and to further support its traditional use as Chinese folk medicine in treatment of urinary infections, especially the disease of prostatitis.
The antinociceptive activity of its water extract (WE), 70% ethanol extract (EE), water eluted part of EE from AB-8 macroporous resin (WEPMR) and 70% ethanol eluted part of EE from AB-8 macroporous resin (EEPMR) was conducted using mice writhing test with different doses. Then the anti-inflammatory activity of the four parts was evaluated on rat models of carrageenan-induced chronic non-bacterial prostatitis (CNP). Preliminary study was taken to determine the phytochemical compositions of the four preparative extracts.
Significant writhing inhibitory effect was found with EE at small (7.5 g/kg body wt.), moderate (15 g/kg body wt.) and large (30 g/kg body wt.) doses (doses here are presented as crude herbs) as well as EEPMR at moderate and large doses by oral administration (OA) (p≤0.01). Data from prostatic index, lecithin microsome density and white blood cell level showed that moderate dose of EE and EEPMR both had significant (p≤0.05 or p≤0.01) inhibition effect on carrageenan-induced inflammation in rat prostate. The HPLC analytical results showed that flavonoids were the main active compounds in WE, EE and EEPMR. And most flavonoids were accumulated into the part of EEPMR by AB-8 macroporous resin leaving only few compounds in WEPMR. No acute toxicity was identified in oral administration of the four parts at a dose of 100g/kg body wt.
The results described here suggest that extracts of the aerial part of Clematis terniflora DC. might be of therapeutic interest in the treatment of prostatitis.
本研究旨在评估铁线莲 DC 的水提物（WE）、70%乙醇提取物（EE）、AB-8 大孔树脂水洗脱部分（WEPMR）和 70%乙醇洗脱部分（EEPMR）的抗炎和镇痛活性，并进一步支持其作为治疗尿路感染的民间药物的传统用途，尤其是前列腺炎的治疗。
采用不同剂量的小鼠扭体试验评价其水提取物（WE）、70%乙醇提取物（EE）、AB-8 大孔树脂水洗脱部分（WEPMR）和 70%乙醇洗脱部分（EEPMR）的镇痛活性。然后，采用角叉菜胶诱导的慢性非细菌性前列腺炎（CNP）大鼠模型评价这四个部分的抗炎活性。初步研究旨在确定四个制备提取物的植物化学成分。
EE 在小（7.5g/kg 体重）、中（15g/kg 体重）和大（30g/kg 体重）剂量以及 EEPMR 在中、大剂量（这里的剂量以粗药表示）时对小鼠扭体有显著的抑制作用（p≤0.01）。前列腺指数、卵磷脂微体密度和白细胞水平的数据表明，EE 和 EEPMR 的中剂量均对大鼠前列腺角叉菜胶诱导的炎症有显著的抑制作用（p≤0.05 或 p≤0.01）。HPLC 分析结果表明，黄酮类化合物是 WE、EE 和 EEPMR 的主要活性化合物。并且，AB-8 大孔树脂将大部分黄酮类化合物吸附到 EEPMR 部分，而 WEPMR 中只留下少量化合物。在 100g/kg 体重的口服剂量下，这四个部分均未表现出急性毒性。
这里描述的结果表明，铁线莲地上部分的提取物可能对前列腺炎的治疗具有治疗意义。
