Miller R L, Rapp F
J Gen Virol. 1977 Sep;36(3):515-24. doi: 10.1099/0022-1317-36-3-515.
Nuclear extracts of varicella-zoster virus (VZV)-infected human embryo lung (HEL) cells were found to contain DNA polymerase activity not present in uninfected HEL cells. This enzyme was designated the VZV-induced DNA polymerase. The VZV-induced polymerase was partially separated from the cellular alpha- and beta-polymerases by fractionation of the cells and by phosphocellulose chromatography. The separated enzymes were examined for the effect of added (NH4)2SO4, activity with synthetic templates, optimal pH, and the effect of phosphonoacetic acid. The VZV-induced DNA polymerase was distinct from cellular enzymes and had the properties of a typical herpesvirus-induced DNA polymerase.
研究发现，水痘带状疱疹病毒（VZV）感染的人胚肺（HEL）细胞的核提取物中含有未感染的HEL细胞所没有的DNA聚合酶活性。这种酶被命名为VZV诱导的DNA聚合酶。通过细胞分级分离和磷酸纤维素层析，VZV诱导的聚合酶与细胞α-和β-聚合酶部分分离。检测分离出的酶对添加硫酸铵（NH4）2SO4的反应、对合成模板的活性、最适pH以及膦酰乙酸的影响。VZV诱导的DNA聚合酶与细胞酶不同，具有典型疱疹病毒诱导的DNA聚合酶的特性。
