Laboratoire AMAPP, EA 4248, Université d'Orléans, Orléans, France.
Horm Metab Res. 2011 Mar;43(3):216-22. doi: 10.1055/s-0030-1269919. Epub 2011 Jan 13.
The aim of the study was to evaluate the effects of short-term glucocorticoid treatment on plasma amino acids, free fatty acids, blood glucose, and several hormones in healthy volunteers performing long-lasting exercise. 9 young female subjects exercised 2 h at 50-55% VO₂ max twice, once after placebo (Pla) ingestion and once after prednisone (Cor, 50 mg/day/7 days) ingestion, according to a double-blind and randomized protocol. Blood samples were tat rest and during exercise for measurement of amino acids, free fatty acids, blood glucose, adrenocorticotrophic hormone (ACTH), growth hormone, dehydroepiandrosterone (DHEA), insulin, and glucagon. Both ACTH and DHEA values were significantly decreased by Cor vs. Pla (p < 0.01) throughout exercise, and Cor intake also induced lower growth hormone concentrations vs. Pla (p < 0.05) from 60 min to the end of exercise. No significant difference in glucagon, insulin or free fatty acid values was found between the treatments. Branched-chain amino acids and other essential amino acids were significantly higher after Cor vs. Pla from 60 min to the end of exercise (p < 0.05), whereas blood glucose was significantly higher from 90 min to the end of exercise (p < 0.05). We conclude that short-term glucocorticoid intake induces marked hormonal and metabolic changes during long-lasting exercise. Proteolysis can increase with glucocorticoid during this type of exercise and the related higher plasma amino acid concentrations may contribute as energy substrates. Further studies will be necessary to explore and accurately quantify the mechanisms of proteolysis and glyconeogenesis induced by short-term glucocorticoid intake during this type of exercise.
本研究旨在评估短期糖皮质激素治疗对进行长时间运动的健康志愿者的血浆氨基酸、游离脂肪酸、血糖和几种激素的影响。9 名年轻女性受试者按照双盲和随机方案，两次进行 2 小时的 50-55%最大摄氧量运动，一次在安慰剂（Pla）摄入后，一次在泼尼松（Cor，50mg/天/7 天）摄入后。在休息和运动期间采集血样，以测量氨基酸、游离脂肪酸、血糖、促肾上腺皮质激素（ACTH）、生长激素、脱氢表雄酮（DHEA）、胰岛素和胰高血糖素。Cor 与 Pla 相比，ACTH 和 DHEA 值在整个运动过程中均显著降低（p<0.01），Cor 摄入还导致生长激素浓度在 60 分钟至运动结束时低于 Pla（p<0.05）。两种处理方式之间的胰高血糖素、胰岛素或游离脂肪酸值无显著差异。与 Pla 相比，Cor 后支链氨基酸和其他必需氨基酸在 60 分钟至运动结束时显著升高（p<0.05），而血糖在 90 分钟至运动结束时显著升高（p<0.05）。我们得出结论，短期糖皮质激素摄入会在长时间运动期间引起明显的激素和代谢变化。在这种类型的运动中，蛋白质分解可能会随着糖皮质激素的增加而增加，相关的较高血浆氨基酸浓度可能作为能量底物。需要进一步的研究来探索和准确量化这种类型的运动期间短期糖皮质激素摄入诱导的蛋白质分解和糖异生的机制。
