Department of Physics, Clarkson University, Potsdam, NY 13699, USA.
Skin Res Technol. 2011 May;17(2):213-9. doi: 10.1111/j.1600-0846.2010.00487.x. Epub 2011 Jan 17.
BACKGROUND/PURPOSE: To develop a modification of the stripping method allowing quick automated processing of corneocyte samples to study human skin conditions. Although the previous scrubbing technique and stripping method can provide sufficient information about human corneocytes, they are either subject to artifacts or involve time-consuming data processing.
In the first stage, an adhesive tape is used to collect corneocytes as in the regular stripping method. Then, a D-squame skin indicator is used to divide the collected corneocytes into a lesser populated sample, in which more individual corneocytes can be observed with the help of fluorescent microscopy after dye staining. The method was applied to study the change of corneocytes after moisturizer (glycerin) treatment.
The modified stripping method described allows the automated processing of the geometrical characteristics of corneocytes. Data for several hundreds of corneocytes can easily be collected. The analysis of glycerin treatment demonstrated a high sensitivity for the method. An average increase of the corneocyte area of 5.1% was found after 9 days of treatment (the accuracy of the method was 0.5%).
The method described is suitable for the automated data processing. It allows for the reliable detection of the expansion of the average area of corneocytes after 9 days of daily glycerin treatment.
背景/目的：开发一种改良的剥落法，允许快速自动处理角质细胞样本，以研究人类皮肤状况。虽然之前的擦洗技术和剥落法可以提供关于人类角质细胞的充分信息，但它们要么容易产生伪影，要么涉及耗时的数据处理。
在第一阶段，使用粘性胶带像常规剥落法一样收集角质细胞。然后，使用 D-squame 皮肤指示剂将收集到的角质细胞分成一个较少的样本，在经过染色后，借助荧光显微镜可以观察到更多的单个角质细胞。该方法用于研究保湿剂（甘油）处理后角质细胞的变化。
描述的改良剥落法允许角质细胞几何特征的自动处理。可以轻松收集数百个角质细胞的数据。对甘油处理的分析显示该方法具有很高的灵敏度。经过 9 天的治疗，发现角质细胞面积平均增加了 5.1%（该方法的准确性为 0.5%）。
所描述的方法适合自动数据处理。它可以可靠地检测到经过 9 天的日常甘油处理后角质细胞平均面积的扩大。
