Department of Infectious Diseases, University of São Paulo, Medical School, São Paulo, Brazil.
Trans R Soc Trop Med Hyg. 2011 Mar;105(3):173-8. doi: 10.1016/j.trstmh.2010.11.010. Epub 2011 Jan 15.
The incidence of cutaneous leishmaniasis (CL) is increasing and there is limited surveillance of Leishmania species throughout the world. We identified the species associated with CL in a region of Amazonia, an area recognized for its Leishmania species variability. Clinical findings were analyzed and correlated with the species identified in 93 patients. PCR assays were based on small subunit ribosomal DNA (SSU-rDNA) and G6PD, and were performed in a laboratory located 3,500km away. Leishmania (V.) braziliensis was identified in 53 patients (57%). The other 40 patients (43%) carried a different species (including six cases of L. (L.) amazonensis). Molecular methods can be employed, using special media, to allow transport to distant laboratories. L. (V.) braziliensis is the most common species in the area of Para. The location of ulcers can suggest CL species.
皮肤利什曼病（CL）的发病率正在上升，而全世界对利什曼原虫物种的监测有限。我们在亚马逊地区确定了与 CL 相关的物种，该地区以其利什曼原虫物种多样性而闻名。对 93 名患者的临床发现进行了分析，并与鉴定出的物种进行了相关性分析。PCR 检测基于小亚基核糖体 DNA（SSU-rDNA）和 G6PD，并在距离 3500 公里的实验室进行。53 名患者（57%）中鉴定出利什曼原虫（V.） braziliensis。其他 40 名患者（43%）携带不同的物种（包括 6 例 L.（L.） amazonensis）。可以使用特殊培养基采用分子方法，允许将标本运送到遥远的实验室。在帕拉地区，L.（V.） braziliensis 是最常见的物种。溃疡的位置可以提示 CL 物种。
