State Key Lab of Rice Biology and Key Lab of the Ministry of Agriculture for Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou, 310029, People's Republic of China.
Funct Integr Genomics. 2011 Mar;11(1):103-10. doi: 10.1007/s10142-011-0209-4. Epub 2011 Jan 18.
Low phytic acid grains can provide a solution to dietary micronutrient deficiency and environmental pollution. A low phytic acid 1-1 (lpa1-1) barley mutant was identified using forward genetics and the mutant gene was mapped to chromosome 2HL. Comparative genomic analysis revealed that the lpa1-1 gene was located in the syntenic region of the rice Os-lpa-MH86-1 gene on chromosome 4. The gene ortholog of rice Os-lpa-MH86-1 (designated as HvST) was isolated from barley using polymerase chain reaction and mapped to chromosome 2HL in a doubled haploid population of Clipper×Sahara. The results demonstrate the collinearity between the rice Os-lpa-MH86-1 gene and the barley lpa1-1 region. Sequence analysis of HvST revealed a single base pair substitution (C→T transition) in the last exon of the gene in lpa1-1 (M422), which resulted in a nonsense mutation. These results will facilitate our understanding of the molecular mechanisms controlling the low phytic acid phenotype and assist in the development of a diagnostic marker for the selection of the lpa1-1 gene in barley.
低植酸谷物可以为解决膳食微量营养素缺乏和环境污染问题提供解决方案。本研究采用正向遗传学方法鉴定了一个低植酸 1-1（lpa1-1）大麦突变体，并将突变基因定位在 2HL 染色体上。比较基因组分析表明，lpa1-1 基因位于水稻 4 号染色体上 Os-lpa-MH86-1 基因的同源区域。利用聚合酶链反应从大麦中分离出水稻 Os-lpa-MH86-1 的基因直系同源物（命名为 HvST），并在 Clipper×Sahara 的双单倍体群体中定位到 2HL 染色体上。这些结果表明水稻 Os-lpa-MH86-1 基因与大麦 lpa1-1 区域之间存在共线性。对 HvST 的序列分析表明，lpa1-1（M422）基因的最后一个外显子中发生了单个碱基对替换（C→T 转换），导致无义突变。这些结果将有助于我们理解控制低植酸表型的分子机制，并有助于开发大麦 lpa1-1 基因选择的诊断标记。
