Jia Lei, Zhao Yanling, Xing Xiaoyan, Wang Jiabo, Zou Wenjun, Li Ruisheng, Yang Hongbo, Cheng Danhong, Xiao Xiaohe
China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(20):2741-4.
To investigate the objectivity and authenticity of the difference of the Cold and Hot nature between Mahuang decoction and Maxingshigan decoction based on the cold/hot plate differentiating assay.
The measuring time and dosage of Mahuang decoction and Maxingshigan decoction were investigated before the compliance experiment. Meanwhile, the activities of adenosine triphosphatase were detected in order to probe into the mechanism of their action.
It was found that the drug dose should be 10 times amount to human dose (0.80 g x kg(-1) of Mahuang decoction and 2.26 g x kg(-1) of Maxing Shigan decoction) and the measuring time should be from 30 min to 60 min after administering. Compared with the control group, the remaining ratio of the mice treated with Mahuang decoction on the hot pad significantly decreased (P < 0.01). Compared with Mahuang decoction, the remaining ratio of the mice treated with Maxingshigan decoction on the hot pad significantly increased (P < 0.05). Compared with the control group, the Na(+) -K(+) -ATPase activity of liver tissue of the mice treated by Mahuang decoction significantly increased (P < 0.05), while the activity of Na(+) -K(+) -ATPase and Mg(2+) -Ca(2+) -ATPase of liver tissue of the mice treated by Maxingshigan decoction reduced significantly (P < 0.05).
This study showed that the differences of Cold and Hot nature of Mahuang decoction and Maxingshigan decoction were objectively existent from animal ethology, which was consistent with the traditional theroy of Chinese medicine. And the changes of ATPase activity of liver tissue might be the mechanism of drug action.
基于寒热板鉴别试验探讨麻黄汤与麻杏石甘汤寒热属性差异的客观性与真实性。
在符合实验前考察麻黄汤与麻杏石甘汤的给药时间及剂量。同时检测三磷酸腺苷酶活性以探讨其作用机制。
发现给药剂量应为人体剂量的10倍（麻黄汤0.80 g·kg⁻¹，麻杏石甘汤2.26 g·kg⁻¹），测量时间应为给药后30分钟至60分钟。与对照组相比，麻黄汤处理的小鼠在热板上的停留比例显著降低（P<0.01）。与麻黄汤相比，麻杏石甘汤处理的小鼠在热板上的停留比例显著升高（P<0.05）。与对照组相比，麻黄汤处理的小鼠肝脏组织的Na⁺-K⁺-ATP酶活性显著升高（P<0.05），而麻杏石甘汤处理的小鼠肝脏组织的Na⁺-K⁺-ATP酶和Mg²⁺-Ca²⁺-ATP酶活性显著降低（P<0.05）。
本研究表明，麻黄汤与麻杏石甘汤寒热属性差异从动物行为学角度客观存在，这与中医传统理论相符。肝脏组织ATP酶活性的变化可能是药物作用机制。
