Zhao Yanling, Jia Lei, Wang Jiabo, Zou Wenjun, Yang Hongbo, Xiao Xiaohe
a Department of Pharmacy , 302 Hospital of People's Liberation Army , Beijing , China .
b School of Chinese Medicine, The University of Hong Kong, Estates Building , Hong Kong , China , and.
Pharm Biol. 2016 Jul;54(7):1298-302. doi: 10.3109/13880209.2015.1057650. Epub 2015 Nov 22.
Chinese medicines with different cold/hot properties have various pharmacological actions on multiple organisms.
The objective of this study was to explore the cold/hot property differences of traditional Chinese medicine formulas of Mahuang and Maxingshigan decoctions.
A novel cold/hot pad differentiating assay method based on the Intelligent Animal Temperature Tropism Behavior monitoring system at 20 °C (cold pad) and 30 °C (hot pad) was introduced to investigate the variability of temperature tropism among the mice treated by 0.4 mL/20 g (drug volume/body weight) of Mahuang decoction and Maxingshigan decoction, respectively. Meanwhile, the oxygen consumption and activities of adenosine triphosphatase (ATPase) were measured to explore the energy metabolism mechanism.
Results showed that the differences between cold/hot properties of Mahuang decoction and Maxingshigan decoction were significant (p < 0.05). Mahuang decoction produced significant synergic effect (a combination index of 1.60), while Maxingshigan decoction expressed significant antagonistic effect (a combination index of 0.35). The changes of energy metabolism including ATPase activity and oxygen consumption might be the possible factors to result in the differences. Those influences tended to be coherent with the definition of cold/hot properties of Chinese medicines based on traditional Chinese medicinal theory.
The results indicated that the method based on cold/hot pad differentiating array could objectively and quantitatively represent the cold/hot properties of different compatibilities of traditional Chinese medicines in an ethological way according to the changes of animal's temperature tropism. These findings would provide some experimental basis and data references as well as a novel evaluation method for the study of the regularity of recipe composition.
具有不同寒/热属性的中药对多种生物体具有多种药理作用。
本研究旨在探讨麻黄汤和麻杏石甘汤这两种中药方剂的寒/热属性差异。
引入一种基于智能动物温度趋向行为监测系统的新型寒/热垫区分测定方法，分别在20℃（寒垫）和30℃（热垫）下，研究以0.4 mL/20 g（药物体积/体重）的麻黄汤和麻杏石甘汤处理的小鼠之间温度趋向性的变化。同时，测量耗氧量和三磷酸腺苷酶（ATPase）活性，以探究能量代谢机制。
结果表明，麻黄汤和麻杏石甘汤的寒/热属性差异显著（p < 0.05）。麻黄汤产生显著的协同作用（联合指数为1.60），而麻杏石甘汤表现出显著的拮抗作用（联合指数为0.35）。包括ATPase活性和耗氧量在内的能量代谢变化可能是导致差异的因素。这些影响倾向于与基于传统中医理论的中药寒/热属性定义相一致。
结果表明，基于寒/热垫区分阵列的方法能够根据动物温度趋向性的变化，以行为学方式客观、定量地反映不同配伍的中药的寒/热属性。这些发现将为方剂组成规律的研究提供一些实验依据和数据参考以及一种新的评价方法。
