Mandel Abigail L, Ozdener Hakan, Utermohlen Virginia
Monell Chemical Senses Center, Philadelphia, PA 19104, USA.
J Immunoassay Immunochem. 2011;32(1):18-30. doi: 10.1080/15321819.2011.538625.
In order to assess the physiological significance of human salivary brain-derived neurotrophic factor (BDNF), we have optimized a sensitive and specific enzyme-linked immunosorbent assay (ELISA). We determined the range of salivary BDNF concentrations, the impact of saliva collection method, and the association of salivary BDNF with several biological characteristics. The ELISA had a detection limit of 62.5 pg/mL, and intra-assay and interassay precisions of 4.2% and 8.2%, respectively. Salivary BDNF concentrations were highly variable between individuals (median = 618 pg/mL) and were affected by collection method. Women had significantly higher levels of salivary BDNF than men. There was no relationship, however, between salivary BDNF levels and the other biological characteristics examined.
为了评估人唾液中脑源性神经营养因子（BDNF）的生理意义，我们优化了一种灵敏且特异的酶联免疫吸附测定（ELISA）方法。我们测定了唾液BDNF浓度范围、唾液采集方法的影响以及唾液BDNF与多种生物学特征的关联。该ELISA的检测限为62.5 pg/mL，批内精密度和批间精密度分别为4.2%和8.2%。个体间唾液BDNF浓度差异很大（中位数 = 618 pg/mL），且受采集方法影响。女性唾液BDNF水平显著高于男性。然而，唾液BDNF水平与所检测的其他生物学特征之间没有关系。
