三七生品和蒸制品及其人参皂苷成分对人肝癌细胞的抗增殖作用。

Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells.

机构信息

Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, 117543, Singapore.

出版信息

Chin Med. 2011 Jan 24;6:4. doi: 10.1186/1749-8546-6-4.

BACKGROUND

Panax notoginseng is a potential source of anticancer compounds. This study aims to investigate the effects of steaming on the chemical profile of P. notoginseng and the anti-proliferative effects of P. notoginseng on liver cancer cells.

METHODS

Samples of powdered raw P. notoginseng roots were steamed for various durations. Extracts of the raw and steamed samples were subjected to ultra-high pressure liquid chromatography/mass spectrometry (UHPLC-MS) analysis for chemical profiling. The anti-proliferative effects on three human liver cancer cells, namely SNU449, SNU182 and HepG2, were evaluated using colorimetric WST-1 assay.

RESULTS

Steaming changed chromatographic and pharmacological profiles of P. notoginseng, causing differences in activities such as inhibition of cancer growth. Steamed P. notoginseng exhibited greater anti-proliferative effects against liver cancer cells (SNU449, SNU182 and HepG2) than its raw form; steaming up to 24 hours increased bioactivities. Steaming increased the concentrations of ginsenoside Rh2, Rk1, Rk3 and 20S-Rg3 and enhanced growth inhibition of liver cancer cells.

CONCLUSION

Steaming changes the chemical profile as well as anti-cancer biological activities of P. notoginseng. Steamed P. notoginseng contains potential compounds for the treatment of liver cancer.

背景

三七是一种有潜力的抗癌化合物来源。本研究旨在探讨蒸制对三七化学成分和三七对肝癌细胞增殖抑制作用的影响。

方法

将粉末状生三七根样品蒸制不同时间。对生样品和蒸制样品的提取物进行超高压液相色谱/质谱（UHPLC-MS）分析以进行化学成分分析。采用比色 WST-1 法评价三种人肝癌细胞（SNU449、SNU182 和 HepG2）的增殖抑制作用。

结果

蒸制改变了三七的色谱和药理特征，导致其活性（如抑制肿瘤生长）的差异。蒸制三七对肝癌细胞（SNU449、SNU182 和 HepG2）的增殖抑制作用强于生三七；蒸制 24 小时以上可提高生物活性。蒸制增加了人参皂苷 Rh2、Rk1、Rk3 和 20S-Rg3 的浓度，并增强了对肝癌细胞的生长抑制作用。