Xiong Yin, Chen Lijuan, Hu Yupiao, Cui Xiuming
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Yunnan Key Laboratory of Panax notoginseng, Kunming, China.
Front Pharmacol. 2017 Oct 18;8:745. doi: 10.3389/fphar.2017.00745. eCollection 2017.
Although (PN) roots in raw and steamed forms were historically supposed to be different in the efficacies, the raw materials and steamed ones were often undifferentiated in the use and market circulation, which might bring unstable curative effects or even adverse reactions. To uncover chemical constituents responsible to different activities of raw and steamed PN, chemometrics analyses including partial least squares regression (PLSR) and multi-linear regression analysis (MLRA) were used to establish the relationships between the chromatographic fingerprints and activities of PN samples. Chemical fingerprints of PN were determined by HPLC. Anticoagulant and antioxidant activities were evaluated by the thromboplastin inhibiting test and hydroxyl radical scavenging assay, respectively. Results showed that there was a significant difference in the chemical composition between raw and steamed PN, which could be discriminated by principle component analysis according to different steaming temperatures. Compared with the steamed PN, raw PN exhibited stronger anticoagulation and weaker antioxidation. By chemometrics analyses, notoginsenoside R, ginsenosides Rg, Re, Rb, and Rd were found to be the major active constituents of raw PN, whereas ginsenosides Rh, Rk, Rh, and 20()-Rg had the key role in the activities of steamed PN, which could be used as new markers for the quality control (QC) of steamed PN.
尽管生三七和蒸制三七在历史上被认为功效不同，但在使用和市场流通中，生熟原料往往没有区分，这可能会带来疗效不稳定甚至不良反应。为了揭示生三七和蒸制三七不同活性的化学成分，采用化学计量学分析方法，包括偏最小二乘回归（PLSR）和多元线性回归分析（MLRA），来建立三七样品色谱指纹图谱与活性之间的关系。三七的化学指纹图谱通过高效液相色谱法测定。抗凝活性和抗氧化活性分别通过凝血活酶抑制试验和羟自由基清除试验进行评估。结果表明，生三七和蒸制三七的化学成分存在显著差异，根据不同的蒸制温度，通过主成分分析可以区分。与蒸制三七相比，生三七表现出更强的抗凝作用和较弱的抗氧化作用。通过化学计量学分析发现，三七皂苷R、人参皂苷Rg、Re、Rb和Rd是生三七的主要活性成分，而人参皂苷Rh、Rk、Rh和20()-Rg在蒸制三七的活性中起关键作用，可作为蒸制三七质量控制（QC）的新指标。
