Arnout J, Huybrechts E, Vanrusselt M, Falcon C, Vermylen J
Centre for Thrombosis and Vascular Research, University of Leuven, Belgium.
Thromb Haemost. 1990 Aug 13;64(1):26-31.
Clotting assays allow qualitative rather than quantitative detection of the lupus anticoagulant. We have therefore studied the usefulness of an ELISA using a commercial partial thromboplastin, Throombofax, as antigen; the results obtained on 146 selected patient plasmas were compared to the results of coagulation tests (kaolin clotting time, tissue thromboplastin inhibition test, activated partial thromboplastin time) and of ELISAs using cardiolipin or phosphatidylserine as antigen. While satisfactory agreement was found within the group of coagulation tests or that of ELISAs, only a moderate agreement was obtained between clotting tests and ELISAs, the best being with the partial thromboplastin ELISA using low plasma dilutions. The study further indicates that ELISA techniques cannot entirely replace coagulation tests for the detection of a lupus anticoagulant, even when a partial thromboplastin is used as antigen. On the other hand, coagulation tests are less sensitive than ELISAs for the detection of antiphospholipid antibodies.
凝血检测只能定性而非定量检测狼疮抗凝物。因此，我们研究了一种以商用部分凝血活酶（Throombofax）作为抗原的酶联免疫吸附测定（ELISA）的实用性；将146份选定患者血浆的检测结果与凝血试验（高岭土凝血时间、组织凝血活酶抑制试验、活化部分凝血活酶时间）以及以心磷脂或磷脂酰丝氨酸作为抗原的ELISA结果进行了比较。虽然在凝血试验组或ELISA组内发现了令人满意的一致性，但凝血试验和ELISA之间仅获得了中等程度的一致性，其中使用低血浆稀释度的部分凝血活酶ELISA一致性最佳。该研究进一步表明，即使使用部分凝血活酶作为抗原，ELISA技术也不能完全替代凝血试验来检测狼疮抗凝物。另一方面，在检测抗磷脂抗体方面，凝血试验不如ELISA敏感。
