NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2916-2921. doi: 10.1099/ijs.0.030130-0. Epub 2011 Jan 21.
Phylogenetic analysis of 'Actinoplanes ianthinogenes' Coronelli et al. 1974 and 'Actinoplanes ianthinogenes subsp. octamycini' Gauze et al. 1979 based on 16S rRNA gene sequencing data revealed that these organisms form a clade in the family Micromonosporaceae. Morphological and chemotaxonomic characteristics of strains of these species were consistent with those of members of the genus Actinoplanes. Morphological, DNA-DNA hybridization, physiological, biochemical and chemotaxonomic data showed that 'A. ianthinogenes' and 'A. ianthinogenes subsp. octamycini' can be easily differentiated from each other and that they merit separate species status. On the basis of morphological, physiological, biochemical, chemotaxonomic and DNA-DNA hybridization data, it is concluded that 'A. ianthinogenes' and 'A. ianthinogenes subsp. octamycini' should be assigned the status of two novel species: Actinoplanes ianthinogenes nom. rev. (type strain NBRC 13996(T)=A/1668(T)=ATCC 21884(T)=BCRC 13611(T)=DSM 43864(T)=IMSNU 20032(T)=JCM 3249(T)=KCTC 9347(T)=KCTC 9592(T)=NCIMB 12639(T)=NRRL B-16720(T)) and Actinoplanes octamycinicus corrig. comb. nov., nom. rev. (type strain NBRC 14524(T)=INA 4041(T)=ATCC 43632(T)=JCM 9649(T)=KCTC 9593(T)), respectively.
基于 16S rRNA 基因测序数据的‘Actinoplanes ianthinogenes’ Coronelli 等人 1974 年和‘Actinoplanes ianthinogenes subsp. octamycini’Gauze 等人 1979 年的系统发育分析表明，这些生物在微单胞菌科中形成一个分支。这些种的菌株的形态学和化学分类学特征与 Actinoplanes 属的成员一致。形态学、DNA-DNA 杂交、生理、生化和化学分类学数据表明，‘A. ianthinogenes’和‘A. ianthinogenes subsp. octamycini’可以很容易地彼此区分开，并且它们值得单独作为物种存在。基于形态学、生理、生化、化学分类学和 DNA-DNA 杂交数据，得出结论，‘A. ianthinogenes’和‘A. ianthinogenes subsp. octamycini’应被指定为两个新种的地位：Actinoplanes ianthinogenes nom. rev.（模式株 NBRC 13996(T)=A/1668(T)=ATCC 21884(T)=BCRC 13611(T)=DSM 43864(T)=IMSNU 20032(T)=JCM 3249(T)=KCTC 9347(T)=KCTC 9592(T)=NCIMB 12639(T)=NRRL B-16720(T)）和 Actinoplanes octamycinicus corrig. comb. nov.，nom. rev.（模式株 NBRC 14524(T)=INA 4041(T)=ATCC 43632(T)=JCM 9649(T)=KCTC 9593(T)）。
