Korean Agricultural Culture Collection, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Microbial Resources Laboratory, School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1813-1823. doi: 10.1099/ijs.0.016451-0.
Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequence analysis showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from other Dactylosporangium type strains and from 'Dactylosporangium salmoneum' NRRL B-16294. Twelve of 13 representative isolates had identical 16S rRNA gene sequences and formed a subclade that was distinct from corresponding phyletic lines composed of the remaining isolate, strain BK63T, the 'D. salmoneum' strain and the type strains of recognized Dactylosporangium species. DNA-DNA relatedness data indicated that representatives of the multi-membered 16S rRNA gene subclade, isolate BK63T and the 'D. salmoneum' subclade formed distinct genomic species; all of these organisms had chemotaxonomic and morphological properties consistent with their classification in the genus Dactylosporangium. They were also distinguished from one another and from the type strains of recognized Dactylosporangium species based on a range of phenotypic properties. Combined genotypic and phenotypic data showed that isolate BK63T, isolates BK51T, BK53 and BK69, and strain NRRL B-16294T should be classified in the genus Dactylosporangium as representing novel species. The names proposed for these species are Dactylosporangium luridum sp. nov. (type strain BK63T =DSM 45324T =KACC 20933T =NRRL B-24775T), Dactylosporangium luteum sp. nov. (type strain BK51T =DSM 45323T =KACC 20899T =NRRL B-24774T) and Dactylosporangium salmoneum sp. nov., nom. rev. (type strain NRRL B-16294T =ATCC 31222T =DSM 43910T =JCM 3272T =NBRC 14103T).
从干草牧场采集的土壤中分离出的 40 株菌基于菌落特性被归为 Dactylosporangium 属。16S rRNA 基因序列分析显示，这些分离株形成的一个群组与 Dactylosporangium aurantiacum 模式菌株最为密切相关，但与其他 Dactylosporangium 模式菌株和“Dactylosporangium salmoneum”NRRL B-16294 明显分离。13 个代表性分离株中的 12 个具有相同的 16S rRNA 基因序列，并形成一个亚分支，与由剩余分离株、菌株 BK63T、“D. salmoneum”菌株和公认的 Dactylosporangium 物种的模式菌株组成的相应系统发育线明显分离。DNA-DNA 相关数据表明，多成员 16S rRNA 基因亚分支的代表、菌株 BK63T 和“D. salmoneum”亚分支形成了不同的基因组种；所有这些生物的化学分类和形态特征都与其在 Dactylosporangium 属中的分类一致。它们还通过一系列表型特性与彼此以及与公认的 Dactylosporangium 物种的模式菌株区分开来。综合基因型和表型数据表明，菌株 BK63T、BK51T、BK53 和 BK69 以及 NRRL B-16294T 应归类为 Dactylosporangium 属，代表新种。为这些物种提出的名称是 Dactylosporangium luridum sp. nov.（模式株 BK63T =DSM 45324T =KACC 20933T =NRRL B-24775T）、Dactylosporangium luteum sp. nov.（模式株 BK51T =DSM 45323T =KACC 20899T =NRRL B-24774T）和 Dactylosporangium salmoneum sp. nov.，nom. rev.（模式株 NRRL B-16294T =ATCC 31222T =DSM 43910T =JCM 3272T =NBRC 14103T）。
