Yamamura Hideki, Miyazaki Shoya, Ikoma Kodai, Nakagawa Youji, Hamada Moriyuki, Otoguro Misa, Tamura Tomohiko, Ando Katsuhiko, Phay Nyunt, Hayakawa Masayuki
Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Japan.
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan.
J Antibiot (Tokyo). 2017 Oct;70(10):995-999. doi: 10.1038/ja.2017.81. Epub 2017 Jul 12.
An actinomycete strain, designated MM04-1133, was isolated from an anthill soil sample collected in Bagan, Myanmar. To establish the taxonomic status of this strain, the isolate was subjected to a polyphasic approach. Strain MM04-1133 was Gram-staining positive, aerobic, motile and formed long and narrow sporangia directly above the surface of the substrate mycelium. Whole-cell hydrolysates of the strain contained 3-OH-diaminopimelic acid, arabinose, glucose, galactose, mannose, rhamnose and xylose. The predominant menaquinones were MK-10(H) and MK-10(H). The major cellular fatty acids were iso-C and anteiso-C. The diagnostic phospholipid was phosphatidylethanolamine. The G+C content of the DNA was 69.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MM04-1133 clustered within the genus Virgisporangium, with the sequence exhibiting highest similarity (98.5% identity) with Virgisporangium ochraceum NBRC 16418. The strain grew in the presence of 0-1% (w/v) NaCl, at pH 5-8 and at 20-40 °C, with optimal growth at 30-37 °C. Based on phylogenetic analysis and chemotaxonomic and phenotypic data, we propose classifying this isolate as a novel species of the genus Virgisporangium, to be designated as Virgisporangium myanmarense sp. nov. The type strain is MM04-1133 (=NBRC 112733=VTCC 910008).
从缅甸蒲甘采集的蚁丘土壤样本中分离出一株放线菌，命名为MM04 - 1133。为确定该菌株的分类地位，采用了多相分类法。菌株MM04 - 1133革兰氏染色阳性，好氧，具运动性，在基内菌丝体表面直接形成狭长的孢子囊。该菌株的全细胞水解产物含有3 - OH - 二氨基庚二酸、阿拉伯糖、葡萄糖、半乳糖、甘露糖、鼠李糖和木糖。主要的甲基萘醌是MK - 10(H)和MK - 10(H)。主要的细胞脂肪酸是异-C和anteiso-C。诊断性磷脂是磷脂酰乙醇胺。DNA的G + C含量为69.1 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株MM04 - 1133聚类于Virgisporangium属内，其序列与Virgisporangium ochraceum NBRC 16418的相似性最高（同一性为98.5%）。该菌株在0 - 1%（w/v）NaCl、pH 5 - 8和20 - 40°C条件下生长，最适生长温度为30 - 37°C。基于系统发育分析、化学分类学和表型数据，我们建议将该分离株归为Virgisporangium属的一个新物种，命名为Virgisporangium myanmarense sp. nov.。模式菌株为MM04 - 1133（=NBRC 112733 = VTCC 910008）。
