K Chu Kengyeh, Lim Daryl, Mertz Jerome
Boston University, Department of Biomedical Engineering 44 Cummington St., Boston, MA, 02215
Biomed Opt Express. 2010 Jul 16;1(1):236-245. doi: 10.1364/BOE.1.000236.
Active illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale. We describe and demonstrate a fully integrated instrument that performs both feedback and image reconstruction. The image is reconstructed on a logarithmic scale to accommodate the dynamic range benefits of AIM in a single output channel. A theoretical and computational analysis of the influence of noise on active illumination feedback is presented, along with imaging examples illustrating the benefits of AIM. While AIM is applicable to any type of scanning microscope, we apply it here specifically to two-photon microscopy.
主动照明显微镜（AIM）是一种在扫描显微镜中重新分配动态范围的方法，它利用实时反馈在亚像素时间尺度上控制照明功率。我们描述并展示了一种执行反馈和图像重建的完全集成仪器。图像在对数尺度上重建，以在单个输出通道中实现AIM的动态范围优势。本文给出了噪声对主动照明反馈影响的理论和计算分析，以及说明AIM优势的成像示例。虽然AIM适用于任何类型的扫描显微镜，但我们在此特别将其应用于双光子显微镜。
