Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
Nat Methods. 2011 Sep 29;8(10):811-9. doi: 10.1038/nmeth.1709.
A key requirement for performing three-dimensional (3D) imaging using optical microscopes is that they be capable of optical sectioning by distinguishing in-focus signal from out-of-focus background. Common techniques for fluorescence optical sectioning are confocal laser scanning microscopy and two-photon microscopy. But there is increasing interest in alternative optical sectioning techniques, particularly for applications involving high speeds, large fields of view or long-term imaging. In this Review, I examine two such techniques, based on planar illumination or structured illumination. The goal is to describe the advantages and disadvantages of these techniques.
使用光学显微镜进行三维（3D）成像是一项关键要求，即能够通过区分聚焦信号和离焦背景来实现光学切片。荧光光学切片的常用技术是共焦激光扫描显微镜和双光子显微镜。但是，人们越来越感兴趣于替代的光学切片技术，特别是对于涉及高速、大视场或长期成像的应用。在这篇综述中，我研究了两种基于平面照明或结构照明的此类技术。目的是描述这些技术的优缺点。
