Gautsch J W, Lerner R, Howard D, Teramoto Y A, Schlom J
J Virol. 1978 Sep;27(3):688-99. doi: 10.1128/JVI.27.3.688-699.1978.
Tryptic peptide analyses were performed on the major structural 52,000- and 36,000-dalton glycoproteins (gp52 and gp36-38) and the nonglycosylated 28,000-, 14,000-, and 10,000-dalton proteins (p28, p14, and p10) of the highly oncogenic murine mammary tumor viruses (MMTVs) of C3H, RIII, and GR mice, i.e., MMTV(C3H), MMTV(RIII), and MMTV(GR), respectively. Each virus was grown in both murine and feline cells to ensure the virus-coded nature of each peptide analyzed. The gp36-38 peptide maps of all three MMTVs were indistinguishable, as were the p14 maps of the different MMTVs. Both the p28 and the gp52 of MMTV(C3H), however, could be clearly distinguished from the corresponding proteins of MMTV(RIII) and MMTV(GR), regardless of whether the viruses were grown in feline or murine cells. The p1o of MMTV(RIII) was clearly different from that of MMTV(C3H) and MMTV(GR). Therefore, tryptic peptide analysis of three proteins, gp52, p28, and p10, can serve to distinguish these three viruses from one another. These studies further characterize the heterogeneity in polypeptides among MMTVs.
对C3H、RIII和GR小鼠的高度致癌性小鼠乳腺肿瘤病毒（MMTV），即分别为MMTV（C3H）、MMTV（RIII）和MMTV（GR）的主要结构52,000道尔顿和36,000道尔顿糖蛋白（gp52和gp36 - 38）以及非糖基化的28,000道尔顿、14,000道尔顿和10,000道尔顿蛋白质（p28、p14和p10）进行了胰蛋白酶肽分析。每种病毒都在小鼠和猫细胞中培养，以确保所分析的每种肽的病毒编码性质。所有三种MMTV的gp36 - 38肽图谱无法区分，不同MMTV的p14图谱也是如此。然而，无论病毒是在猫细胞还是小鼠细胞中培养，MMTV（C3H）的p28和gp52都能与MMTV（RIII）和MMTV（GR）的相应蛋白质明显区分开来。MMTV（RIII）的p10与MMTV（C3H）和MMTV（GR）的p10明显不同。因此，对gp52、p28和p10这三种蛋白质进行胰蛋白酶肽分析可用于区分这三种病毒。这些研究进一步表征了MMTV之间多肽的异质性。
