Daniel T M, Good R C, Janicki B W
Am Rev Respir Dis. 1975 Nov;112(5):639-44. doi: 10.1164/arrd.1975.112.5.639.
Immunoelectrophoretic analyses were performed on a cell extract and culture filtrate of Mycobacterium tuberculosis, H37Rv strain using homologous polyvalent goat antisera. General similarity between the cell extract and culture filtrate antigens was found. Significant variation between two anti-culture filtrate antisera was observed. These antigens and antisera also differed significantly from previously described reference reagents. These findings demonstrate the necessity of using reference materials for precise identification of mycobacterial antigens in comparative studies.
使用同源多价山羊抗血清对结核分枝杆菌H37Rv菌株的细胞提取物和培养滤液进行了免疫电泳分析。发现细胞提取物和培养滤液抗原之间存在一般相似性。观察到两种抗培养滤液抗血清之间存在显著差异。这些抗原和抗血清也与先前描述的参考试剂有显著差异。这些发现表明，在比较研究中使用参考材料对于精确鉴定分枝杆菌抗原是必要的。
