Univ.-Clinic for Anesthesia, Anichstrasse 35, 6020 Innsbruck, Austria.
Cancer Biomark. 2010;7(3):153-61. doi: 10.3233/CBM-2010-0182.
Analysis of volatile organic compounds (VOCs) provides an elegant approach for cancer screening and disease monitoring, whose use is currently limited by a lack of validated cancer-derived metabolites, which may serve as biomarkers. The aim of the experiments presented here was to investigate the release and consumption of VOCs from the non small cell lung cancer cell line NCI-H1666, which was originally derived from a bronchoalveolar carcinoma.Following detachment by trypsinization suspended cells were incubated in a sealed fermenter for 21 hours. 200 ml of headspace from the cell culture were sampled, diluted with dry, highly purified air and preconcentrated by adsorption on three different solid sorbents with increasing adsorption strength. VOC-analysis was performed by thermodesorption-gas chromatography mass spectrometry (TD-GC-MS). In contrast to our previous studies experiments with NCI-H1666 cells only confirmed the consumption of several aldehydes, n-butyl acetate and the ethers methyl tert-butyl ether and ethyl tert-butyl ether, but no unequivocal release of VOCs was observed. Together with our previously published work these data indicate that the consumption of certain VOCs is commonly observed while their release shows cell line-restricted patterns, whose underlying causes are unknown.
分析挥发性有机化合物 (VOCs) 为癌症筛查和疾病监测提供了一种优雅的方法，但目前由于缺乏经过验证的癌症衍生代谢物作为生物标志物，其应用受到限制。本实验旨在研究非小细胞肺癌细胞系 NCI-H1666 释放和消耗 VOCs 的情况，该细胞系最初源自支气管肺泡癌。用胰蛋白酶消化使悬浮细胞脱离后，将其在密封发酵罐中孵育 21 小时。从细胞培养物中采集 200ml 顶空，用干燥、高纯度空气稀释，并通过在三种具有不同吸附强度的固体吸附剂上吸附进行预浓缩。采用热解吸-气相色谱-质谱联用技术 (TD-GC-MS) 进行 VOC 分析。与我们之前的研究相比，NCI-H1666 细胞实验仅证实了几种醛、正丁酯以及甲基叔丁基醚和乙基叔丁基醚的消耗，但未观察到明确的 VOC 释放。结合我们之前发表的工作，这些数据表明，某些 VOC 的消耗是常见的，而它们的释放则表现出细胞系受限的模式，其潜在原因尚不清楚。
