Department of Operative Medicine, Center of Operative Medicine, Innsbruck Medical University, Innsbruck, Austria.
Cancer Epidemiol Biomarkers Prev. 2010 Jan;19(1):182-95. doi: 10.1158/1055-9965.EPI-09-0162.
The aim of this study was to confirm the existence of volatile organic compounds (VOC) specifically released or consumed by the lung cancer cell line A549, which could be used in future screens as biomarkers for the early detection of lung cancer. For comparison, primary human bronchial epithelial cells (HBEpC) and human fibroblasts (hFB) were included. VOCs were detected in the headspace of cell cultures or medium controls following adsorption on solid sorbents, thermodesorption, and analysis by gas chromatography mass spectrometry. Using this approach, we identified VOCs that behaved similarly in normal and transformed cells. Thus, concentrations of 2-pentanone and 2,4-dimethyl-1-heptene were found to increase in the headspace of A549, HBEpC, and hFB cell cultures. In addition, the ethers methyl tert-butyl ether and ethyl tert-butyl ether could be detected at elevated levels in the case of A549 cells and one of the untransformed cell lines. However, especially branched hydrocarbons and alcohols were seen increased more frequently in untransformed than A549 cells. A big variety of predominantly aldehydes and the ester n-butyl acetate were found at decreased concentrations in the headspace of all cell lines tested compared with medium controls. Again, more different aldehydes were found to be decreased in hFB and HBEpC cells compared with A549 cells and 2-butenal was metabolized exclusively by both control cell lines. These data suggest that certain groups of VOCs may be preferentially associated with the transformed phenotype.
本研究旨在确认肺癌细胞系 A549 特异性释放或消耗的挥发性有机化合物 (VOC) 的存在，这些 VOC 未来可作为肺癌早期检测的生物标志物进行筛选。为了进行比较，还纳入了原代人支气管上皮细胞 (HBEpC) 和人成纤维细胞 (hFB)。在吸附于固体吸附剂、热解吸并通过气相色谱质谱分析后，检测细胞培养物或培养基对照物的顶空处的 VOC。通过这种方法，我们鉴定了在正常和转化细胞中行为相似的 VOC。因此，在 A549、HBEpC 和 hFB 细胞培养物的顶空处，发现 2-戊酮和 2,4-二甲基-1-庚烯的浓度增加。此外，在 A549 细胞和一种未转化的细胞系中，可以检测到醚甲基叔丁基醚和乙基叔丁基醚的浓度升高。然而，与未转化的细胞相比，特别是支链烃和醇在未转化的细胞中更频繁地增加。与培养基对照相比，在所有测试的细胞系的顶空处发现主要为醛类的大量挥发性有机物和酯类 n-丁酸乙酯的浓度降低。同样，与 A549 细胞相比，hFB 和 HBEpC 细胞中发现更多不同的醛类降低，2-丁烯醛仅由两种对照细胞系代谢。这些数据表明，某些 VOC 可能与转化表型密切相关。
