用于点状物体远场三维纳米尺度定位的螺旋点扩展函数。
Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects.
机构信息
Department of Electrical Engineering, Stanford University, 350 Serra Mall, Stanford, California 94305, USA.
出版信息
Opt Lett. 2011 Jan 15;36(2):202-4. doi: 10.1364/OL.36.000202.
Abstract
We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 μm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z.
摘要
我们描述了一种螺旋点扩散函数（PSF），它可以在 3.2μm 的景深范围内以纳米精度定位三维物体。螺旋 PSF 随发射器的轴向（z）位置而旋转。Fisher 信息计算表明，螺旋 PSF 可以用有限数量的光子实现纳米级定位精度。我们通过在三角形聚二甲基硅氧烷（PDMS）光栅表面成像珠子来演示了具有螺旋 PSF 的三维超分辨率显微镜。在检测到 99,000 个光子后，螺旋 PSF 在 x 方向的定位精度为 2.7nm，在 y 方向的定位精度为 2.1nm，在 z 方向的定位精度为 5.7nm。
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