Laboratory of Genital Tract Biology, Department of Obstetrics and Gynecology, Harvard Medical School and Brigham and Women’s Hospital, Boston, Massachusetts, USA.
mBio. 2011 Jan 18;2(1):e00280-10. doi: 10.1128/mBio.00280-10.
The fetal response to intrauterine inflammatory stimuli appears to contribute to the onset of preterm labor as well as fetal injury, especially affecting newborns of extremely low gestational age. To investigate the role of placental colonization by specific groups of microorganisms in the development of inflammatory responses present at birth, we analyzed 25 protein biomarkers in dry blood spots obtained from 527 newborns delivered by Caesarean section in the 23rd to 27th gestation weeks. Bacteria were detected in placentas and characterized by culture techniques. Odds ratios for having protein concentrations in the top quartile for gestation age for individual and groups of microorganisms were calculated. Mixed bacterial vaginosis (BV) organisms were associated with a proinflammatory pattern similar to those of infectious facultative anaerobes. Prevotella and Gardnerella species, anaerobic streptococci, peptostreptococci, and genital mycoplasmas each appeared to be associated with a different pattern of elevated blood levels of inflammation-related proteins. Lactobacillus was associated with low odds of an inflammatory response. This study provides evidence that microorganisms colonizing the placenta provoke distinctive newborn inflammatory responses and that Lactobacillus may suppress these responses.
胎儿对宫内炎症刺激的反应似乎有助于早产的发生以及胎儿损伤，特别是对极低胎龄的新生儿影响较大。为了研究特定微生物群体在出生时炎症反应发展中的胎盘定植作用，我们分析了 527 例在第 23 至 27 孕周行剖宫产分娩的新生儿的干血斑中的 25 种蛋白质生物标志物。通过培养技术检测胎盘中的细菌并对其进行了特征描述。针对个体微生物和微生物群体，我们计算了胎龄蛋白浓度处于最高四分位数的比值比。混合细菌性阴道病（BV）微生物与类似感染兼性厌氧菌的促炎模式相关。普雷沃氏菌属和加德纳菌属、厌氧链球菌、消化链球菌和生殖支原体似乎与炎症相关蛋白血液水平升高的不同模式相关。乳酸杆菌与炎症反应的低几率相关。这项研究提供了证据，表明定植于胎盘的微生物会引发新生儿独特的炎症反应，而乳酸杆菌可能会抑制这些反应。
