School of Engineering and Technology, University of Hertfordshire, College Lane, Hatfield, Hertfordshire AL10 9AB, United Kingdom.
Biomicrofluidics. 2010 Dec 23;4(4):44112. doi: 10.1063/1.3528327.
We have previously reported that microthrottle pumps (MTPs) display the capacity to pump solid phase suspensions such as polystyrene beads which prove challenging to most microfluidic pumps. In this paper we report employing a linear microthrottle pump (LMTP) to pump whole, undiluted, anticoagulated, human venous blood at 200 μl min(-1) with minimal erythrocyte lysis and no observed pump blockage. LMTPs are particularly well suited to particle suspension transport by virtue of their relatively unimpeded internal flow-path. Micropumping of whole blood represents a rigorous real-world test of cell suspension transport given blood's high cell content by volume and erythrocytes' relative fragility. A modification of the standard Drabkin method and its validation to spectrophotometrically quantify low levels of erythrocyte lysis by hemoglobin release is also reported. Erythrocyte lysis rates resulting from transport via LMTP are determined to be below one cell in 500 at a pumping rate of 102 μl min(-1).
我们之前曾报道过，微节流泵（MTP）能够泵送固相悬浮液，如聚苯乙烯珠，这对大多数微流控泵来说极具挑战性。在本文中，我们报告了使用线性微节流泵（LMTP）以 200μl/min 的速度泵送完整、未稀释、抗凝的人静脉血，红细胞裂解最小，没有观察到泵阻塞。LMTP 特别适合于颗粒悬浮液的输送，因为它们具有相对通畅的内部流道。全血的微量泵送代表了对细胞悬浮液输送的严格的真实世界测试，因为血液的细胞含量高，红细胞相对脆弱。还报告了对标准 Drabkin 方法的修改及其验证，以通过血红蛋白释放分光光度法定量测定低水平的红细胞裂解。通过 LMTP 输送导致的红细胞裂解率在泵送速率为 102μl/min 时低于每 500 个细胞一个。
