Fan Junqiang, Xu Shiwei, Chen Fang, Ding Minjun
Department of Cardiothoracic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Jun;25(3):632-6.
Hemolysis in blood pumps has been measured by various in vitro tests, in which normalized index of hemolysis (NIH) was established. As NIH is complicated and difficult to calculate, erythrocyte fragment count (EFC) is proposed in the present study to predict hemolysis in roller pumps. Five paired in vitro tests were conducted using the POLYSTAN pediatric pump (group A) and COBE pump (group B). Ten whole blood samples (400ml) were circled in the roller pump for 16 hours. Erythrocyte fragments count and plasma-free hemoglobin (FHb) were measured before pumping and every 2 hours through circulation after 4 hours pumping. The morphological changes of erythrocyte were observed by scanning electron microscope. Simple linear regression analysis showed the two groups' EFC and FHb levels increased linearly during a long period of pumping, and the coefficient of determination (R2) was 0.64. These data suggested that Erythrocyte fragments count could be used as an index in evaluating the in vitro hemolytic properties of blood pumps.
血液泵中的溶血已通过各种体外试验进行测量，其中建立了标准化溶血指数（NIH）。由于NIH复杂且难以计算，本研究提出用红细胞碎片计数（EFC）来预测滚压泵中的溶血情况。使用POLYSTAN儿科泵（A组）和COBE泵（B组）进行了五项配对体外试验。将十份全血样本（400毫升）在滚压泵中循环16小时。在泵血前以及泵血4小时后每2小时循环一次后测量红细胞碎片计数和无血浆血红蛋白（FHb）。通过扫描电子显微镜观察红细胞的形态变化。简单线性回归分析表明，在长时间泵血过程中，两组的EFC和FHb水平呈线性增加，决定系数（R2）为0.64。这些数据表明，红细胞碎片计数可作为评估血液泵体外溶血特性的一个指标。
