Iida K, Hase K, Shimomura K, Sudo S, Kadota S, Namba T
Mikimoto Pharmaceutical Co., Ltd., Ise, Japan.
Planta Med. 1995 Oct;61(5):425-8. doi: 10.1055/s-2006-958129.
Thirty-three crude drug extracts were screened for their tyrosinase inhibitory activity. Among them, the acetone extract of the rhizomes of Rheum officinale Baillon showed the strongest inhibitory activity. Tyrosinase inhibitory activity-guided fractionation and chemical analysis led to the isolation of two potent compounds, 3,4',5-trihydroxystilbene-4'-O-beta-D-(2"-O-galloyl)glucopyr anoside (1) and 3,4',5-trihydroxystilbene-4'-O-beta-D-(6"-O-galloyl)glucopyr anoside (2). These compounds showed a competitive inhibition against tyrosinase and also inhibited the melanin biosynthesis.
对33种粗提物进行了酪氨酸酶抑制活性筛选。其中，掌叶大黄根茎的丙酮提取物显示出最强的抑制活性。通过酪氨酸酶抑制活性导向的分级分离和化学分析，分离出两种有效化合物，3,4',5-三羟基茋-4'-O-β-D-(2''-O-没食子酰基)吡喃葡萄糖苷(1)和3,4',5-三羟基茋-4'-O-β-D-(6''-O-没食子酰基)吡喃葡萄糖苷(2)。这些化合物对酪氨酸酶表现出竞争性抑制作用，并且还抑制黑色素生物合成。
