用于 DNA 预浓缩的荷电选择性纳米孔水凝胶的光流体型无掩模原位光刻。
Optofluidic in situ maskless lithography of charge selective nanoporous hydrogel for DNA preconcentration.
出版信息
Biomicrofluidics. 2010 Dec 30;4(4):43014. doi: 10.1063/1.3516037.
Abstract
An optofluidic maskless photopolymerization process was developed for in situ negatively charged nanoporous hydrogel [poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid)] fabrication. The optofluidic maskless lithography system, which combines a high power UV source and digital mirror device, enables fast polymerization of arbitrary shaped hydrogels in a microfluidic device. The poly-AMPS hydrogel structures were positioned near the intersections of two microchannels, and were used as a cation-selective filter for biological sample preconcentration. Preconcentration dynamics as well as the fabricated polymer shape were analyzed in three-dimensions using fluorescein sample and a confocal microscope. Finally, single-stranded DNA preconcentration was demonstrated for polymerase chain reaction-free signal enhancement.
摘要
开发了一种用于原位带负电荷纳米多孔水凝胶 [聚-AMPS（2-丙烯酰胺基-2-甲基-1-丙磺酸）] 制造的光流控无掩模光聚合工艺。光流控无掩模光刻系统结合了高功率 UV 源和数字微镜器件，可在微流控设备中快速聚合任意形状的水凝胶。聚 AMPS 水凝胶结构位于两个微通道的交汇处附近，用作生物样品预浓缩的阳离子选择性过滤器。使用荧光素样品和共聚焦显微镜在三维空间中分析了预浓缩动力学和所制造的聚合物形状。最后，通过无聚合酶链反应信号增强演示了单链 DNA 的预浓缩。
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