Koistinaho J, Yang G
Department of Public Health, University of Tampere, Finland.
Histochemistry. 1990;95(1):73-6. doi: 10.1007/BF00737230.
Induction of c-fos protein (FOS) after the onset of darkness was studied immunocytochemically in the rat and hamster pineal gland. The animals were kept on a 12:12 h light-dark cycle. Before the dark period no FOS staining was seen in either rat or hamster pineal cells. Five hours after the onset of darkness 342 +/- 18 pinealocytes/0.2 mm2 (mean +/- SD) displayed FOS-like immunoreactivity in the hamster pineal gland; in the rat pineal gland only 5 +/- 2 pinealocytes/0.2 mm2 showed a faint staining. Two hours later the density of FOS positive cells was decreased to 60 +/- 11/0.2 mm2 in the hamster but increased to 519 +/- 103/0.2 mm2 in the rat pineal gland. Three hours before the beginning of the light period no FOS positive cells were detected in either animal. Both the rat and hamster pineal gland showed a transient and temporally defined expression of c-fos protein in the middle of the dark period. This may be related to a more active functional state of pinealocytes, which is reflected in a peak of melatonin synthesis during the darkness.
采用免疫细胞化学方法研究了大鼠和仓鼠松果体在黑暗开始后c-fos蛋白（FOS）的诱导情况。动物饲养于12:12小时明暗循环条件下。在黑暗期开始前，大鼠和仓鼠的松果体细胞均未见FOS染色。黑暗开始5小时后，仓鼠松果体中342±18个松果体细胞/0.2 mm²（平均值±标准差）呈现FOS样免疫反应性；大鼠松果体中仅5±2个松果体细胞/0.2 mm²显示微弱染色。两小时后，仓鼠中FOS阳性细胞密度降至60±11/0.2 mm²，而大鼠松果体中则增至519±103/0.2 mm²。在光照期开始前3小时，两种动物的松果体均未检测到FOS阳性细胞。大鼠和仓鼠的松果体在黑暗期中间均呈现c-fos蛋白的短暂且有时间限定的表达。这可能与松果体细胞更活跃的功能状态有关，黑暗期间褪黑素合成高峰反映了这种状态。
