Faculdade de Farmácia/CEF, Universidade de Coimbra, Azinhaga de Santa Comba, 3030-548 Coimbra, Portugal.
Exp Parasitol. 2011 Apr;127(4):732-9. doi: 10.1016/j.exppara.2011.01.011. Epub 2011 Jan 25.
The present work evaluates the anti-Giardia activity of Syzygium aromaticum and its major compound eugenol. The effects were evaluated on parasite growth, adherence, viability and ultrastructure. S. aromaticum essential oil (IC(50)=134 μg/ml) and eugenol (IC(50)=101 μg/ml) inhibited the growth of G. lamblia. The essential oil inhibited trophozoites adherence since the first hour of incubation and was able to kill almost 50% of the parasites population in a time dependent manner. The eugenol inhibited G. lamblia trophozoites adherence since the third hour and not induce cell lyses. The main morphological alterations were modifications on the cell shape, presence of precipitates in the cytoplasm, autophagic vesicles, internalization of flagella and ventral disc, membrane blebs, and intracellular and nuclear clearing. Taken together, our findings lead us to propose that eugenol was responsible for the anti-giardial activity of the S. aromaticum essential oil and both have potential for use as therapeutic agents against giardiasis.
本研究评估了丁香及其主要化合物丁香酚的抗贾第虫活性。评估了其对寄生虫生长、黏附、活力和超微结构的影响。丁香精油（IC50=134μg/ml）和丁香酚（IC50=101μg/ml）抑制了 G. lamblia 的生长。精油在孵育的第一小时就抑制了滋养体的黏附，并且能够以时间依赖的方式杀死近 50%的寄生虫种群。丁香酚自孵育第 3 小时开始抑制 G. lamblia 滋养体的黏附，且不会诱导细胞裂解。主要的形态学改变包括细胞形状的改变、细胞质中沉淀物的存在、自噬小泡、鞭毛和腹盘的内化、质膜泡、细胞内和核清除。综上所述，我们的研究结果表明，丁香酚是丁香精油抗贾第虫活性的原因，二者均有作为抗贾第虫病治疗药物的潜力。
