Medical University of Lublin, Chair and Department of Biology and Genetics, Staszica 6, Lublin 20-081, Poland.
Exp Parasitol. 2011 Jun;128(2):163-5. doi: 10.1016/j.exppara.2011.01.007. Epub 2011 Jan 25.
It is known that highly reactive oxygene species produced during normal cellular metabolism represent a powerful effector mechanism against parasites. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) belong to the main defense anti-oxidants that prevent the formation of new free radical species. The aim of this study was to assess the activities of SOD and GPx in cattle tissues infected with Taenia saginata. We observed a statistically significant increase in the SOD and GPx activities (p=0.00003, 0.00008, respectively, Student's t-test) in skeletal muscles infected with T. saginata in spectrophotometric analysis. With the use of western blot technique, SOD synthesis stimulation has appeared in the host tissues containing cysticerci in contrast with the control samples. There was no statistically significant increase in the GPx band intensity observed in the studied samples in comparison to controls (Gene Tools Version 4.01 program). These results support the significance of anti-oxidant processes in host defense mechanism during parasitic infections.
已知在正常细胞代谢过程中产生的高反应性氧物种代表了一种针对寄生虫的强大效应机制。超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）属于主要的防御抗氧化剂，可防止新自由基的形成。本研究旨在评估感染有囊尾蚴的牛组织中的 SOD 和 GPx 活性。我们在分光光度分析中观察到，感染有囊尾蚴的骨骼肌中的 SOD 和 GPx 活性（分别为 p=0.00003，0.00008，Student's t 检验）有统计学意义的增加。使用 Western blot 技术，在含有囊尾蚴的宿主组织中观察到 SOD 合成的刺激，与对照样品相比。与对照相比，在研究样本中观察到的 GPx 带强度没有统计学意义的增加（Gene Tools Version 4.01 程序）。这些结果支持抗氧化过程在寄生虫感染宿主防御机制中的重要性。
