Department of Ophthalmology, Affiliated Hospital of Nantong University, Nantong, China.
Ophthalmic Res. 2011;46(2):80-7. doi: 10.1159/000323179. Epub 2011 Jan 26.
To investigate the effectiveness and feasibility of inducing myopia in mice by flickering-light (FL) stimulation.
Forty-five 28-day-old C57BL/6 (B6) mice were randomly assigned to three groups: control group, FL stimulation group and form deprivation (FD) group. Mice in the control group were raised under 250 lux illumination from 8:00 a.m. to 8:00 p.m. Mice in the FL group were raised under illumination with a duty cycle of 50% at a flash rate of 2 Hz from 8:00 a.m. to 8:00 p.m. for 6 weeks. Mice in the FD group were raised under the same conditions as the control group; the right eyes of the mice were covered with semitransparent hemispherical plastic shells serving as eye diffusers. The refractive state and axial length (AL) of the right eyes were measured by eccentric infrared photorefraction and A-scan ultrasonography, respectively, before treatment and after 2, 4, 6 or 8 weeks' treatment.
After 6 weeks' exposure to FL, the refraction became more myopic compared with the control group as indicated by longer AL compared with the control group (p < 0.05); the FD eyes were more myopic than the FL eyes (p < 0.05). However, some mice lost their eye diffusers, and lens opacities were found.
Myopia can be induced by FL in B6 mice. The myopic shift induced by FL is less than that induced by FD, but FL causes fewer side effects, and is safery and easier to manipulate.
探讨闪光光刺激（FL）诱导小鼠近视的有效性和可行性。
将 45 只 28 日龄 C57BL/6（B6）小鼠随机分为 3 组：对照组、FL 刺激组和形觉剥夺（FD）组。对照组小鼠在 8:00-20:00 期间接受 250 lux 的照明，FL 组小鼠在 8:00-20:00 期间接受 50%占空比、闪光频率为 2 Hz 的照明，持续 6 周。FD 组小鼠在与对照组相同的条件下饲养；右眼用半透明的半球形塑料壳覆盖作为眼罩。在治疗前和治疗后 2、4、6 或 8 周时，通过偏心红外屈光折射和 A 扫描超声分别测量右眼的屈光状态和眼轴（AL）。
FL 暴露 6 周后，与对照组相比，AL 更长，提示折射更近视（p<0.05）；FD 眼比 FL 眼更近视（p<0.05）。然而，一些小鼠丢失了眼罩，并且发现晶状体混浊。
FL 可诱导 B6 小鼠发生近视。FL 诱导的近视漂移小于 FD 诱导的近视漂移，但 FL 引起的副作用更少，更安全且更易于操作。
