从一名患有伯基特淋巴瘤的美国患者的组织中分离出一种疱疹病毒特异性DNA聚合酶。
Isolation of a herpesvirus-specific DNA polymerase from tissues of an American patient with Burkitt lymphoma.
作者信息
Allaudeen H S, Bertino J R
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4504-8. doi: 10.1073/pnas.75.9.4504.
A DNA polymerase (DNA nucleotidyltransferase) has been partially purified from a neck mass of an American patient with Burkitt lymphoma and separated from the cellular DNA polymerases. The molecular weight of the enzyme was approximately 90,000. The enzyme differs from the cellular DNA polymerases, but resembles herpes-virus-induced DNA polymerase in its primer template preference, high monovalent cation requirement for activity, and sensitivity to phosphonoacetate. Enzyme activity was inhibited specifically by an antibody directed against herpes-simplex-virus-induced DNA polymerase but not by antibodies directed against DNA polymerase alpha of HeLa cells and DNA polymerase gamma of a normal human lymphoblast cell line, NC37. Although serum of the patient with Burkitt lymphoma contained high Epstein-Barr virus titer, addition of the serum to the assay mixture did not have any effect on the activity of Burkitt lymphoma DNA polymerase. Tissues from spleen and liver of the patient with Burkitt lymphoma did not contain the herpes-virus-induced DNA polymerase. Detection of the herpes virus polymerase in the Burkitt lymphoma tissue provides additional evidence for the association of Epstein-Barr virus with this malignancy.
从一名患有伯基特淋巴瘤的美国患者的颈部肿块中部分纯化出一种DNA聚合酶（DNA核苷酸转移酶），并将其与细胞DNA聚合酶分离。该酶的分子量约为90,000。该酶与细胞DNA聚合酶不同，但在引物模板偏好、对活性的高单价阳离子需求以及对膦乙酸的敏感性方面类似于疱疹病毒诱导的DNA聚合酶。针对单纯疱疹病毒诱导的DNA聚合酶的抗体可特异性抑制酶活性，但针对HeLa细胞的DNA聚合酶α和正常人淋巴母细胞系NC37的DNA聚合酶γ的抗体则无此作用。尽管伯基特淋巴瘤患者的血清中爱泼斯坦-巴尔病毒滴度很高，但将血清添加到测定混合物中对伯基特淋巴瘤DNA聚合酶的活性没有任何影响。伯基特淋巴瘤患者脾脏和肝脏的组织中不含疱疹病毒诱导的DNA聚合酶。在伯基特淋巴瘤组织中检测到疱疹病毒聚合酶为爱泼斯坦-巴尔病毒与这种恶性肿瘤的关联提供了额外证据。
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