Fabre M, Vong R, Gaillard T, Merens A, Gérome P, Saint-Blancard P, Mechaï F, Janvier F, Nouridjan F, Soler C
Service de biologie médicale, HIA Percy, 101, avenue H.-Barbusse, 92141 Clamart, France.
Pathol Biol (Paris). 2011 Feb;59(1):26-8. doi: 10.1016/j.patbio.2010.08.007. Epub 2011 Jan 31.
The purpose of this study was to evaluate the SD Bioline Ag MPT64 Rapid(®) for identification of the Mycobacterium tuberculosis complex. The method uses an immunochromatographic assay and needs 100 μl of sample taken from liquid culture or colonies suspended. The sensitivity was determined using 99 strains of M. tuberculosis complex and the specificity using 10 nontuberculous mycobacteria and 85 strains other than mycobacteria genus. The test showed excellent sensitivity (99%) and specificity (100%). This technique displays several advantages and is destined to spread in all laboratories and particularly in endemic areas.
本研究的目的是评估SD Bioline Ag MPT64 Rapid(®)用于鉴定结核分枝杆菌复合群的效果。该方法采用免疫层析测定法，需要从液体培养物或悬浮菌落中采集100μl样本。使用99株结核分枝杆菌复合群菌株测定敏感性，使用10株非结核分枝杆菌和85株非分枝杆菌属菌株测定特异性。该检测显示出优异的敏感性（99%）和特异性（100%）。这项技术具有若干优势，注定会在所有实验室中推广，尤其是在流行地区。
