Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea.
J Ethnopharmacol. 2011 Apr 12;134(3):703-10. doi: 10.1016/j.jep.2011.01.022. Epub 2011 Jan 26.
Cordyceps militaris is a well-known medicinal mushroom used for treatment of asthma, and other bronchial and lung inflammatory diseases.
To investigate the anti-inflammatory effects and mechanism of Cordyceps militaris extract on a murine model of acute colitis.
We induced colitis using DSS for 1 week. The disease activity index (DAI) took into account body weight loss, diarrhea, and bleeding. Colon length and crypt length were measured using a microscope. Structural changes of the colon were observed by H&E staining. NO, iNOS, and TNF-α were determined using the Griess assay. iNOS protein was determined using western blotting and quantitative reverse-transcriptase polymerase chain reaction, respectively. Degranulated mast cells in colon tissue were stained using toluidine blue. The degree of degranulated RBL-2H3 cells was measured by the β-hexosaminidase assay.
Cordyceps militaris extract significantly attenuated DSS-induced DAI scores (e.g., body weight loss, diarrhea, gross bleeding). Cordyceps militaris extract also effectively prevented shortening of colon length and crypt length. Histological analysis indicated that Cordyceps militaris extract suppressed epithelial damage, loss of goblet cells, loss of crypts, and infiltration of inflammatory cells induced by DSS. In addition, Cordyceps militaris extract inhibited iNOS and TNF-α mRNA expression in colon tissue of DSS-induced colitis and in LPS-stimulated RAW264.7 cells. Cordyceps militaris extract suppressed degranulation of mast cells in the colon of mice with DSS-induced colitis and in antigen-stimulated mast cells.
These results suggest that Cordyceps militaris extract has anti-inflammatory activity in DSS-induced acute colitis by down-regulating production and expression of inflammatory mediators. These findings suggest that Cordyceps militaris extract might be applied as an agent for prevention or treatment of inflammatory bowel diseases (IBDs).
蛹虫草是一种广为人知的药用真菌，用于治疗哮喘和其他支气管及肺部炎症性疾病。
研究蛹虫草提取物对急性结肠炎小鼠模型的抗炎作用及机制。
我们使用 DSS 诱导结肠炎 1 周。疾病活动指数（DAI）考虑了体重减轻、腹泻和出血。使用显微镜测量结肠长度和隐窝长度。通过 H&E 染色观察结肠结构变化。使用 Griess 测定法测定 NO、iNOS 和 TNF-α。使用 Western blot 和定量逆转录聚合酶链反应分别测定 iNOS 蛋白。使用甲苯胺蓝染色检测结肠组织中脱颗粒肥大细胞。通过β-己糖胺酶测定法测量 RBL-2H3 细胞脱颗粒程度。
蛹虫草提取物显著减轻 DSS 诱导的 DAI 评分（如体重减轻、腹泻、肉眼出血）。蛹虫草提取物还能有效防止结肠长度和隐窝长度缩短。组织学分析表明，蛹虫草提取物抑制 DSS 诱导的结肠炎和 LPS 刺激的 RAW264.7 细胞中上皮损伤、粘蛋白细胞丢失、隐窝丢失和炎症细胞浸润。此外，蛹虫草提取物抑制 DSS 诱导的结肠炎和 LPS 刺激的 RAW264.7 细胞中 iNOS 和 TNF-αmRNA 表达。蛹虫草提取物抑制 DSS 诱导的结肠炎小鼠结肠肥大细胞脱颗粒和抗原刺激的肥大细胞脱颗粒。
这些结果表明，蛹虫草提取物通过下调炎症介质的产生和表达，对 DSS 诱导的急性结肠炎具有抗炎活性。这些发现表明，蛹虫草提取物可作为预防或治疗炎症性肠病（IBD）的药物。
