Key Laboratory of Marine Bio-Resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2011 Dec;61(Pt 12):2967-2973. doi: 10.1099/ijs.0.030205-0. Epub 2011 Jan 29.
A novel endophytic actinomycete, strain YIM 65646(T), was isolated from the roots of Artemisia annua L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10-40 °C (optimum 20-28 °C) and at pH 6.0-9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646(T) belonged to the family Micromonosporaceae (sharing ≤93.6% sequence similarity with members of this family) and formed a distinct clade in the Micromonosporaceae phylogenetic tree. The strain contained meso-diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H(4)), MK-10(H(2)), MK-8(H(2)), MK-9(H(2)) and MK-10(H(6)). The major fatty acids were iso-C(15:0), anteiso-C(15:0), anteiso-C(17:0), iso-C(17:0) and iso-C(16:0). The DNA G+C content of strain YIM 65646(T) was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, Phytomonospora endophytica gen. nov., sp. nov., in the family Micromonosporaceae. The type strain is YIM 65646(T) (=CCTCC AA 209041(T) =DSM 45386(T)).
一株新型内生放线菌，菌株 YIM 65646(T)，从中国西南云南省采集的青蒿根部分离得到。该菌在 10-40°C 的温度范围内生长（最佳温度为 20-28°C），pH 值为 6.0-9.0（最佳 pH 值为 7.0）。该菌形成发育良好的分支基质菌丝，但不产生气生菌丝。表型特征和 16S rRNA 基因序列分析表明，菌株 YIM 65646(T)属于 Micromonosporaceae 科（与该科成员的序列相似度≤93.6%），并在 Micromonosporaceae 系统发育树中形成一个独特的分支。该菌株的细胞壁含有 meso-二氨基庚二酸，全细胞水解物中含有甘露糖、核糖、半乳糖和葡萄糖。主要的类异戊二烯醌为 MK-10(H(4))、MK-10(H(2))、MK-8(H(2))、MK-9(H(2))和 MK-10(H(6))。主要脂肪酸为 iso-C(15:0)、anteiso-C(15:0)、anteiso-C(17:0)、iso-C(17:0)和 iso-C(16:0)。菌株 YIM 65646(T)的 DNA G+C 含量为 70.0 mol%。基于形态学和化学分类学特征以及基于 16S rRNA 基因序列数据的系统发育分析，建议该菌株应归类于新属和新种，即内生植物单胞菌属 Phytomonospora endophytica gen. nov.，sp. nov.，属于 Micromonosporaceae 科。模式菌株为 YIM 65646(T)=CCTCC AA 209041(T)=DSM 45386(T)。
