Key Laboratory of Marine Bio-resources Sustainable Utilization CAS, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, PR China.
Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):224-229. doi: 10.1099/ijs.0.035725-0. Epub 2012 Mar 2.
Three filamentous actinomycetes, strains YIM 65594(T), YIM 65638 and YIM 65642, were isolated from the surface-sterilized roots of Artemisia annua L. collected from Yunnan province, south-west China. These strains were found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. The organisms formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores. The cell wall of the isolates contained ll-diaminopimelic acid and the menaquinones were MK-9(H(8)) and MK-9(H(6)). The major fatty acids were anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0). Phylogenetic analysis of the 16S rRNA gene sequences of these strains revealed that the strains clustered together and were most closely related to Streptomyces kunmingensis NBRC 14463(T), with 98.5-98.6 % 16S rRNA gene sequence similarity. The results of DNA-DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strains YIM 65594(T), YIM 65638 and YIM 65642 from related species. However, the high level of DNA-DNA relatedness between them showed that these three strains belong to the same species. Strain YIM 65594(T) (= DSM 41984(T) = CCTCC AA 209036(T)) was selected as the type strain to represent this novel species, for which the name Streptomyces endophyticus sp. nov. is proposed.
三株丝状放线菌，菌株 YIM 65594(T)、YIM 65638 和 YIM 65642，从中国西南云南省采集的已消毒的青蒿根部分离得到。这些菌株具有典型链霉菌属的形态和化学分类学特征。这些生物形成广泛分枝的基质菌丝，有丰富的气生菌丝分化成孢子。分离物的细胞壁含有 ll-二氨基庚二酸，并且menaquinones 是 MK-9(H(8)) 和 MK-9(H(6))。主要脂肪酸是anteiso-C(15 : 0)、anteiso-C(17 : 0)和 iso-C(16 : 0)。这些菌株的 16S rRNA 基因序列的系统发育分析表明，这些菌株聚在一起，与 Streptomyces kunmingensis NBRC 14463(T)最为密切相关，16S rRNA 基因序列相似度为 98.5-98.6%。DNA-DNA 杂交和生理试验的结果允许在表型和基因型上区分菌株 YIM 65594(T)、YIM 65638 和 YIM 65642 与相关物种。然而，它们之间高水平的 DNA-DNA 相关性表明这三个菌株属于同一物种。菌株 YIM 65594(T) (=DSM 41984(T)=CCTCC AA 209036(T)) 被选为代表该新种的模式菌株，提议命名为内生链霉菌。
