Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2652-2658. doi: 10.1099/ijs.0.016477-0. Epub 2009 Dec 30.
An actinomycete strain, K07-0523(T), was isolated from the roots of an orchid collected in Okinawa prefecture, Japan. 16S rRNA gene sequence analysis indicated that the new strain belonged to the family Micromonosporaceae and the similarity values between strain K07-0523(T) and the type species of 24 genera in the family Micromonosporaceae were 93.3-97.7 %. Strain K07-0523(T) contained D-glutamic acid, glycine, D-alanine, meso-diaminopimelic acid, hydroxydiaminopimelic acid and L-lysine in the cell wall. The major menaquinones were MK-9(H(6)), MK-10(H(4)) and MK-10(H(6)). Galactose, glucose, mannose, ribose and xylose were present in the whole-cell sugars. The acyl type of the peptidoglycan was glycolyl. Major fatty acids were anteiso-C(17 : 0), iso-C(17 : 0), iso-C(16 : 0) and iso-C(15 : 0). Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II. The G+C content of the genomic DNA was 73 mol%. On the basis of phylogenetic and chemotaxonomic data, the new strain represents a member of a new genus and novel species, namely Phytohabitans suffuscus gen. nov., sp. nov., in the family Micromonosporaceae. The type strain of the type species is K07-0523(T) (=DSM 45306(T)=NBRC 105367(T)).
一株放线菌菌株 K07-0523(T) 从日本冲绳县采集的兰花根部分离得到。16S rRNA 基因序列分析表明，该新菌株属于微单胞菌科，与该科 24 个属的模式种的相似度值为 93.3-97.7%。K07-0523(T) 菌株的细胞壁含有 D-谷氨酸、甘氨酸、D-丙氨酸、meso-二氨基庚二酸、羟基二氨基庚二酸和 L-赖氨酸。主要的menaquinones 是 MK-9(H(6))、MK-10(H(4))和 MK-10(H(6))。全细胞糖中含有半乳糖、葡萄糖、甘露糖、核糖和木糖。肽聚糖的酰基类型为甘醇酰基。主要脂肪酸为anteiso-C(17 : 0)、iso-C(17 : 0)、iso-C(16 : 0)和 iso-C(15 : 0)。磷脂酰乙醇胺被检测为主要的磷脂，并对应于磷脂 II 型。基因组 DNA 的 G+C 含量为 73 mol%。基于系统发育和化学分类学数据，该新菌株代表了微单胞菌科中新属和新种的成员，即 Phytohabitans suffuscus 属，新种，模式菌株为 K07-0523(T) (=DSM 45306(T)=NBRC 105367(T))。
