School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
Antonie Van Leeuwenhoek. 2013 Feb;103(2):399-408. doi: 10.1007/s10482-012-9820-1. Epub 2012 Sep 26.
A novel endophytic actinomycete, designated strain NEAU-J3(T), was isolated from soybean root (Glycine max (L.) Merr) and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain NEAU-J3(T) fell within the family Micromonosporaceae. The strain was observed to form an extensively branched substrate mycelium, which carried non-motile oval spores with a smooth surface. The cell walls of strain NEAU-J3(T) were determined to contain meso-diaminopimelic acid and galactose, ribose and glucose were detected as whole-cell sugars. The major menaquinones were determined to be MK-9(H(4)) and MK-9(H(6)). The phospholipids detected were phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were determined to be C(16:0), C(18:1) ω9c, C(18:0), C(17:0), C(17:1) ω7c, anteiso-C(17:0), C(16:1) ω7c and C(15:0). The DNA G + C content was 62.5 mol%. On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-J3(T) is considered to represent a novel species of a new genus within the family Micromonosporaceae, for which the name Wangella harbinensis gen. nov., sp. nov. is proposed. The type strain of Wangella harbinensis is strain NEAU-J3(T) (=CGMCC 4.7039(T) = DSM 45747(T)).
一株新的内生放线菌，命名为 NEAU-J3(T) 菌株，从大豆根（Glycine max (L.) Merr）中分离得到，并通过多相方法进行了表征。基于 16S rRNA 基因序列的系统发育分析表明，NEAU-J3(T) 菌株属于 Micromonosporaceae 科。该菌株形成广泛分支的基质菌丝，产生无动力的椭圆形孢子，表面光滑。NEAU-J3(T) 菌株的细胞壁含有meso-二氨基庚二酸和半乳糖，全细胞糖检测到核糖和葡萄糖。主要的menaquinones 确定为 MK-9(H(4)) 和 MK-9(H(6))。检测到的磷脂为磷脂酰胆碱和磷脂酰乙醇胺。主要的细胞脂肪酸确定为 C(16:0)、C(18:1) ω9c、C(18:0)、C(17:0)、C(17:1) ω7c、anteiso-C(17:0)、C(16:1) ω7c 和 C(15:0)。DNA G+C 含量为 62.5 mol%。基于形态学和化学分类学特征、系统发育分析以及 16S rRNA 基因特征核苷酸的特征模式，NEAU-J3(T) 菌株被认为代表了 Micromonosporaceae 科一个新属的新种，因此提议建立一个新属 Wangella，新种 Wangella harbinensis gen. nov.，sp. nov.。Wangella harbinensis 的模式菌株是 NEAU-J3(T) 菌株（=CGMCC 4.7039(T)=DSM 45747(T)）。
