Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Section for Experimental Oncology and Nanomedicine, Else Kröner-Fresenius-Stiftung, Waldstr. 1, 91054 Erlangen, Germany.
Histochem Cell Biol. 2011 Feb;135(2):153-8. doi: 10.1007/s00418-011-0780-8. Epub 2011 Jan 29.
In order to increase the dose of antineoplastic agents in the tumor area, the concept of magnetic drug targeting (MDT) has been developed. Magnetic nanoparticles consisting of iron oxide and a biocompatible cover layer suspended in an aqueous solution (ferrofluid) serve as carriers for chemotherapeutics being enriched by an external magnetic field after intra-arterial application in desired body compartments (i.e., tumor). We established an ex vivo model to simulate in vivo conditions in a circulating system consisting of magnetic iron oxide nanoparticles passing an intact bovine artery and being focused by an external magnetic field to study their distribution in the vessel. Micro-computed X-ray tomography (XμCT) and histology can elucidate the arrangement of these particles after application. XμCT-analysis has been performed on arterial sections after MDT in order to determine the distribution of the nanoparticles. These measurements have been carried out with a cone X-ray source and corresponding histological sections were stained with Prussian blue. It could be shown that combining XμCT and histology offers the opportunity for a better understanding of the mechanisms of nanoparticle deposition in the vascular system after MDT.
为了增加肿瘤区域内抗肿瘤药物的剂量，开发了磁药物靶向（MDT）的概念。由氧化铁和生物相容性覆盖层组成的磁性纳米粒子悬浮在水溶液中（磁流体），用作化学治疗剂的载体，在动脉内应用于所需的体腔（即肿瘤）后，通过外部磁场富集。我们建立了一个体外模型来模拟由通过完整牛动脉的磁性氧化铁纳米粒子组成的循环系统中的体内条件，并通过外部磁场将其聚焦，以研究它们在血管中的分布。微计算机断层扫描（XμCT）和组织学可以阐明应用后这些粒子的排列。为了确定纳米粒子的分布，对 MDT 后的动脉切片进行了 XμCT 分析。这些测量是使用锥形 X 射线源进行的，相应的组织学切片用普鲁士蓝染色。结果表明，结合 XμCT 和组织学为更好地了解 MDT 后纳米粒子在血管系统中的沉积机制提供了机会。
