视色素在蟾蜍（海蟾蜍）视杆细胞和鲶鱼（斑点叉尾鮰）视锥细胞中的侧向扩散。
Lateral diffusion of visual pigments in toad (Bufo marinus) rods and in catfish (Ictalurus punctatus) cones.
作者信息
Gupta B D, Williams T P
机构信息
Institute of Molecular Biophysics, Florida State University, Tallahassee 32306.
出版信息
J Physiol. 1990 Nov;430:483-96. doi: 10.1113/jphysiol.1990.sp018303.
Abstract
- The lateral diffusion coefficient, D, was determined for visual pigments in red rods, green rods and red-sensitive cones with a photon-counting microspectrophotometer. 2. A novel protocol that involved the placement on a photoreceptor of a single micromeasuring/bleaching beam permitted the determination of D of the pigment. 3. Demonstration that D for red rods compared very well with values obtained by other workers using other means validated the protocol. 4. Applied to green rods, the protocol gave a value of D that was about 80% greater than that for red rods. 5. D for cone pigment was found to be slightly less than that of red rod pigment. 6. The dichroic ratio of cones and the average orientation of the chromophore in the cone lamellae were 1.71 and 28.4 deg, respectively. 7. The photosensitivity at lambda max of the red-sensitive cones was found to be about 16% less than that of red rods.
摘要
- 使用光子计数显微分光光度计测定了红色视杆细胞、绿色视杆细胞和红色敏感视锥细胞中视觉色素的横向扩散系数D。2. 一种新的实验方案，即将单个微测量/漂白光束置于光感受器上，可用于测定色素的D值。3. 红色视杆细胞的D值与其他研究人员使用其他方法获得的值非常吻合，这证明了该实验方案的有效性。4. 将该实验方案应用于绿色视杆细胞时，得到的D值比红色视杆细胞的D值大约高80%。5. 发现视锥色素的D值略小于红色视杆色素的D值。6. 视锥细胞的二向色性比率和视锥细胞薄片中发色团的平均取向分别为1.71和28.4度。7. 发现红色敏感视锥细胞在最大波长处的光敏性比红色视杆细胞低约16%。
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